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Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue.

Authors :
Naaijkens BA
Krijnen PA
Meinster E
ter Horst EN
Vo K
Musters RJ
Kamp O
Niessen HW
Juffermans LJ
van Dijk A
Source :
Cell and tissue research [Cell Tissue Res] 2015 Dec; Vol. 362 (3), pp. 623-32. Date of Electronic Publication: 2015 Jul 23.
Publication Year :
2015

Abstract

In most pre-clinical animal studies investigating stem cell therapy in acute myocardial infarction (AMI), the administered stem cells are isolated from healthy donors. In clinical practice, however, patients who suffer from AMI will receive autologous cells, for example using adipose-derived stem cells (ASC). During AMI, inflammation is induced and we hypothesized that this might affect characteristics of ASC. To investigate this, ASC were isolated from rat adipose tissue 1 day (1D group, n = 5) or 7 days (7D group, n = 6) post-AMI, and were compared with ASC from healthy control rats (Control group, n = 6) and sham-operated rats (Sham 1D group, n = 5). We found that significantly fewer ASC were present 1 day post-AMI in the stromal vascular fraction (SVF), determined by a colony-forming-unit assay (p < 0.001 vs. Control and 7D). These data were confirmed by flow cytometry, showing fewer CD90-positive cells in SVF of the 1D group. When cultured, no differences were found in proliferation rate and cell size between the groups in the first three passages. Also, no difference in the differentiation capacity of ASC was found. In conclusion, it was shown that significantly fewer stem cells were present in the SVF 1 day post-AMI; however, the stem cells that were present showed no functional differences.

Details

Language :
English
ISSN :
1432-0878
Volume :
362
Issue :
3
Database :
MEDLINE
Journal :
Cell and tissue research
Publication Type :
Academic Journal
Accession number :
26202892
Full Text :
https://doi.org/10.1007/s00441-015-2239-z