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Genotoxicity evaluation of benzene, di(2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate using a combined rat comet/micronucleus assays.
Genotoxicity evaluation of benzene, di(2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate using a combined rat comet/micronucleus assays.
- Source :
-
Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2015 Jul; Vol. 786-788, pp. 137-43. Date of Electronic Publication: 2015 May 08. - Publication Year :
- 2015
-
Abstract
- As a part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo alkaline comet assay (comet assay), we examined DNA damage in the liver, stomach, and bone marrow of rats dosed orally three times with up to 2000 mg/kg of benzene, di(2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate. All three compounds gave negative results in the liver and stomach. In addition, a bone marrow comet and micronucleus analysis revealed that benzene, but not di(2-ethylhexyl) phthalate or trisodium ethylenediamine tetraacetic acid monohydrate induced a significant increase in the median % tail DNA and micronucleated polychromatic erythrocytes, compared with the respective concurrent vehicle control. These results were in good agreement with the previously reported genotoxicity findings for each compound. The present study has shown that combining the micronucleus test with the comet assay and carrying out these analyses simultaneously is effective in clarifying the mechanism of action of genotoxic compounds such as benzene.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bone Marrow drug effects
DNA Fragmentation drug effects
Dose-Response Relationship, Drug
Liver drug effects
Male
Rats
Rats, Sprague-Dawley
Stomach drug effects
Benzene toxicity
Comet Assay methods
DNA Damage drug effects
Diethylhexyl Phthalate toxicity
Edetic Acid toxicity
Micronucleus Tests methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3592
- Volume :
- 786-788
- Database :
- MEDLINE
- Journal :
- Mutation research. Genetic toxicology and environmental mutagenesis
- Publication Type :
- Academic Journal
- Accession number :
- 26212304
- Full Text :
- https://doi.org/10.1016/j.mrgentox.2015.05.002