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Assessment of CD27 expression as a tool for active and latent tuberculosis diagnosis.
- Source :
-
The Journal of infection [J Infect] 2015 Nov; Vol. 71 (5), pp. 526-33. Date of Electronic Publication: 2015 Aug 05. - Publication Year :
- 2015
-
Abstract
- Unlabelled: There are still no reliable tests to distinguish active tuberculosis (TB) from latent TB infection (LTBI). Assessment of CD27 modulation on CD4⁺ T-cells has been suggested as a tool to diagnose different TB stages.<br />Objectives: To use several cytometric approaches to evaluate CD27 expression on Mycobacterium tuberculosis (Mtb)-specific CD4⁺ T-cells to differentiate TB stages.<br />Methods: 55 HIV-uninfected subjects were enrolled: 13 active TB; 12 cured TB; 30 LTBI. Whole blood was stimulated with RD1-proteins or Cytomegalovirus-lysate (CMV). Interferon (IFN)-γ response was evaluated by cytometry. The proportion of CD27(±) within the IFN-γ⁺ CD4⁺ T-cells or RATIO of the CD27-median fluorescence intensity (MFI) of CD4⁺ T-cells over the CD27 MFI of IFN-γ⁺ CD4⁺ T-cells was evaluated.<br />Results: The greatest diagnostic accuracy in discriminating active TB vs. LTBI or cured TB was reached by evaluating the CD27(+) CD45RA(-) cells within the IFN-γ⁺ CD4⁺ T-cell subset (76.92 sensitivity for both, and 90% and 91.67% specificity, respectively), although the use of the CD27 MFI RATIO allows for stricter data analysis, independent of the operator.<br />Conclusions: the study of CD27 expression using different approaches, whether it involves evaluation of CD45RA expression or not, is a robust biomarker for discriminating TB stages.<br /> (Copyright © 2015 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Antigens, Bacterial immunology
Biomarkers
Female
Flow Cytometry
Humans
Interferon-gamma blood
Latent Tuberculosis immunology
Leukocyte Common Antigens analysis
Leukocyte Common Antigens genetics
Male
Middle Aged
Mycobacterium tuberculosis immunology
Sensitivity and Specificity
Tumor Necrosis Factor Receptor Superfamily, Member 7 genetics
Young Adult
CD4-Positive T-Lymphocytes immunology
Latent Tuberculosis diagnosis
Tuberculosis diagnosis
Tumor Necrosis Factor Receptor Superfamily, Member 7 analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2742
- Volume :
- 71
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of infection
- Publication Type :
- Academic Journal
- Accession number :
- 26253021
- Full Text :
- https://doi.org/10.1016/j.jinf.2015.07.009