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Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo.
- Source :
-
Cell reports [Cell Rep] 2015 Aug 18; Vol. 12 (7), pp. 1205-16. Date of Electronic Publication: 2015 Aug 06. - Publication Year :
- 2015
-
Abstract
- The Piwi-interacting RNA (piRNA) pathway plays an essential role in the repression of transposons in the germline. Other functions of piRNAs such as post-transcriptional regulation of mRNAs are now emerging. Here, we perform iCLIP with the PIWI protein Aubergine (Aub) and identify hundreds of maternal mRNAs interacting with Aub in the early Drosophila embryo. Gene expression profiling reveals that a proportion of these mRNAs undergo Aub-dependent destabilization during the maternal-to-zygotic transition. Strikingly, Aub-dependent unstable mRNAs encode germ cell determinants. iCLIP with an Aub mutant that is unable to bind piRNAs confirms piRNA-dependent binding of Aub to mRNAs. Base pairing between piRNAs and mRNAs can induce mRNA cleavage and decay that are essential for embryonic development. These results suggest general regulation of maternal mRNAs by Aub and piRNAs, which plays a key developmental role in the embryo through decay and localization of mRNAs encoding germ cell determinants.<br /> (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Drosophila embryology
Drosophila genetics
Drosophila metabolism
Drosophila Proteins metabolism
Germ Cells cytology
Peptide Initiation Factors metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Small Interfering metabolism
Drosophila Proteins genetics
Gene Expression Regulation, Developmental
Germ Cells metabolism
Peptide Initiation Factors genetics
RNA Stability
RNA, Small Interfering genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 12
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 26257181
- Full Text :
- https://doi.org/10.1016/j.celrep.2015.07.030