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Protein Labelling with Versatile Phosphorescent Metal Complexes for Live Cell Luminescence Imaging.
- Source :
-
Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2015 Sep 28; Vol. 21 (40), pp. 14146-55. Date of Electronic Publication: 2015 Aug 11. - Publication Year :
- 2015
-
Abstract
- To take advantage of the luminescent properties of d(6) transition metal complexes to label proteins, versatile bifunctional ligands were prepared. Ligands that contain a 1,2,3-triazole heterocycle were synthesised using Cu(I) catalysed azide-alkyne cycloaddition "click" chemistry and were used to form phosphorescent Ir(III) and Ru(II) complexes. Their emission properties were readily tuned, by changing either the metal ion or the co-ligands. The complexes were tethered to the metalloprotein transferrin using several conjugation strategies. The Ir(III)/Ru(II)-protein conjugates could be visualised in cancer cells using live cell imaging for extended periods without significant photobleaching. These versatile phosphorescent protein-labelling agents could be widely applied to other proteins and biomolecules and are useful alternatives to conventional organic fluorophores for several applications.<br /> (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Details
- Language :
- English
- ISSN :
- 1521-3765
- Volume :
- 21
- Issue :
- 40
- Database :
- MEDLINE
- Journal :
- Chemistry (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 26264214
- Full Text :
- https://doi.org/10.1002/chem.201501630