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Role of adenosine 5'-monophosphate-activated protein kinase in α-linolenic acid-induced intestinal lipid metabolism.
- Source :
-
The British journal of nutrition [Br J Nutr] 2015 Sep 28; Vol. 114 (6), pp. 866-72. Date of Electronic Publication: 2015 Aug 13. - Publication Year :
- 2015
-
Abstract
- n-3 Long-chain PUFA up-regulate intestinal lipid metabolism. However, whether these metabolic effects of PUFA on intestine are mediated by AMP-activated protein kinase (AMPK) remains to be elucidated. To determine the effects of α-linolenic acid (ALA) on intestinal fatty acid (FA) metabolism and whether these effects were affected by AMPK deletion, mice deficient in the catalytic subunit of AMPKα1 or AMPKα2 and wild-type (WT) mice were fed either a high-fat diet (HF) or HF supplemented with ALA (HF-A). The results showed that ALA supplementation decreased serum TAG content in WT mice. ALA also increased mRNA expression of genes (carnitine palmitoyltransferase 1a, acyl-CoA oxidase 1, medium-chain acyl-CoA dehydrogenase, cytochrome P450 4A10 and pyruvate dehydrogenase kinase isoenzyme 4a) involved in intestinal lipid oxidation and mRNA expression of TAG synthesis-related genes (monoacylglycerol O-acyltransferase 2, diacylglycerol O-acyltransferases 1 and 2) in WT mice. Consistent with these, expression levels of phosphorylated AMPKα1 and AMPKα2 were also increased in WT mice after ALA addition. However, in the absence of either AMPKα1 or AMPKα2, ALA supplementation failed to increase intestinal lipid oxidation. In addition, no significant effects of either diet (HF and HF-A) or genotype (WT, AMPKα1(-/-) and AMPKα2(-/-)) on FA uptake in the intestine and faecal TAG output were observed. Our results suggest that AMPK is indispensable for the effects of ALA on intestinal lipid oxidation.
- Subjects :
- AMP-Activated Protein Kinases genetics
Animals
Diet, High-Fat adverse effects
Dietary Supplements
Feces chemistry
Hypertriglyceridemia blood
Hypertriglyceridemia etiology
Hypertriglyceridemia metabolism
Hypertriglyceridemia prevention & control
Ileum enzymology
Ileum metabolism
Intestinal Mucosa enzymology
Jejunum enzymology
Jejunum metabolism
Male
Mice, Inbred C57BL
Mice, Knockout
Phosphorylation
Protein Processing, Post-Translational
Triglycerides adverse effects
Triglycerides analysis
Triglycerides blood
Triglycerides metabolism
AMP-Activated Protein Kinases metabolism
Enzyme Induction
Intestinal Mucosa metabolism
Lipid Metabolism
Up-Regulation
alpha-Linolenic Acid therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2662
- Volume :
- 114
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 26268732
- Full Text :
- https://doi.org/10.1017/S0007114515002391