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Functional RelBE-Family Toxin-Antitoxin Pairs Affect Biofilm Maturation and Intestine Colonization in Vibrio cholerae.
- Source :
-
PloS one [PLoS One] 2015 Aug 14; Vol. 10 (8), pp. e0135696. Date of Electronic Publication: 2015 Aug 14 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Toxin-antitoxin (TA) systems are small genetic elements that typically encode a stable toxin and its labile antitoxin. These cognate pairs are abundant in prokaryotes and have been shown to regulate various cellular functions. Vibrio cholerae, a human pathogen that is the causative agent of cholera, harbors at least thirteen TA loci. While functional HigBA, ParDE have been shown to stabilize plasmids and Phd/Doc to mediate cell death in V. cholerae, the function of seven RelBE-family TA systems is not understood. In this study we investigated the function of the RelBE TA systems in V. cholerae physiology and found that six of the seven relBE loci encoded functional toxins in E. coli. Deletion analyses of each relBE locus indicate that RelBE systems are involved in biofilm formation and reactive oxygen species (ROS) resistance. Interestingly, all seven relBE loci are induced under the standard virulence induction conditions and two of the relBE mutants displayed a colonization defect, which was not due to an effect on virulence gene expression. Although further studies are needed to characterize the mechanism of action, our study reveals that RelBE systems are important for V. cholerae physiology.
- Subjects :
- Animals
Bacterial Proteins genetics
Cholera genetics
Cholera metabolism
Humans
Mice
Microbial Viability
Mutation
Virulence Factors genetics
Bacterial Proteins metabolism
Biofilms growth & development
Gene Expression Regulation physiology
Intestines microbiology
Vibrio cholerae physiology
Virulence Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26275048
- Full Text :
- https://doi.org/10.1371/journal.pone.0135696