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CsBAFF, a Teleost B Cell Activating Factor, Promotes Pathogen-Induced Innate Immunity and Vaccine-Induced Adaptive Immunity.
- Source :
-
PloS one [PLoS One] 2015 Aug 21; Vol. 10 (8), pp. e0136015. Date of Electronic Publication: 2015 Aug 21 (Print Publication: 2015). - Publication Year :
- 2015
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Abstract
- B cell activating factor (BAFF) is a member of the tumor necrosis factor family that is known to play an important role in B cell activation, proliferation, and differentiation in mammals. However, studies of BAFF in teleosts are very limited and its function, in particular that under in vivo conditions, is essentially unknown. In this study, we conducted in vivo as well as in vitro functional analyses of a BAFF homologue (CsBAFF) from the teleost fish tongue sole (Cynoglossus semilaevis). CsBAFF is composed of 261 residues and shares moderate sequence identities with known BAFFs of other teleosts. CsBAFF expression was most abundant in immune organs and was upregulated during bacterial infection. Purified recombinant CsBAFF (rCsBAFF) bound to tongue sole lymphocytes and promoted cellular proliferation and survival. The results of an in vivo study showed that CsBAFF overexpression in tongue sole significantly enhanced macrophage activation and reduced bacterial infection in fish tissues, whereas knockdown of CsBAFF expression resulted in increased bacterial dissemination and colonization in fish tissues. Furthermore, vaccination studies showed that CsBAFF enhanced the immunoprotection of a DNA vaccine and augmented the production of specific serum antibodies. Taken together, these results provide the first in vivo evidence to indicate that teleost BAFF is an immunostimulator that significantly contributes to the innate antibacterial immune response and vaccine-induced adaptive immune response.
- Subjects :
- Adaptive Immunity
Amino Acid Sequence
Animals
Antibodies, Bacterial blood
B-Cell Activation Factor Receptor genetics
B-Lymphocytes drug effects
B-Lymphocytes immunology
B-Lymphocytes microbiology
Cell Proliferation
Cell Survival immunology
Edwardsiella tarda immunology
Enterobacteriaceae Infections immunology
Enterobacteriaceae Infections microbiology
Enterobacteriaceae Infections prevention & control
Fish Diseases immunology
Fish Diseases microbiology
Fish Proteins genetics
Gene Expression Regulation
Immunity, Innate
Macrophage Activation drug effects
Macrophages drug effects
Macrophages immunology
Macrophages microbiology
Molecular Sequence Data
Recombinant Proteins genetics
Recombinant Proteins immunology
Sequence Alignment
Sequence Homology, Amino Acid
B-Cell Activation Factor Receptor immunology
Bacterial Vaccines administration & dosage
Enterobacteriaceae Infections veterinary
Fish Diseases prevention & control
Fish Proteins immunology
Flatfishes immunology
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26295165
- Full Text :
- https://doi.org/10.1371/journal.pone.0136015