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Death-associated Protein 3 Regulates Mitochondrial-encoded Protein Synthesis and Mitochondrial Dynamics.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2015 Oct 09; Vol. 290 (41), pp. 24961-74. Date of Electronic Publication: 2015 Aug 25. - Publication Year :
- 2015
-
Abstract
- Mitochondrial morphologies change over time and are tightly regulated by dynamic machinery proteins such as dynamin-related protein 1 (Drp1), mitofusion 1/2, and optic atrophy 1 (OPA1). However, the detailed mechanisms of how these molecules cooperate to mediate fission and fusion remain elusive. DAP3 is a mitochondrial ribosomal protein that involves in apoptosis, but its biological function has not been well characterized. Here, we demonstrate that DAP3 specifically localizes in the mitochondrial matrix. Knockdown of DAP3 in mitochondria leads to defects in mitochondrial-encoded protein synthesis and abnormal mitochondrial dynamics. Moreover, depletion of DAP3 dramatically decreases the phosphorylation of Drp1 at Ser-637 on mitochondria, enhancing the retention time of Drp1 puncta on mitochondria during the fission process. Furthermore, autophagy is inhibited in the DAP3-depleted cells, which sensitizes cells to different types of death stimuli. Together, our results suggest that DAP3 plays important roles in mitochondrial function and dynamics, providing new insights into the mechanism of a mitochondrial ribosomal protein function in cell death.<br /> (© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Subjects :
- Animals
Apoptosis Regulatory Proteins deficiency
Apoptosis Regulatory Proteins genetics
Autophagy
Cell Line
Dynamins chemistry
Dynamins metabolism
Gene Knockdown Techniques
Gene Knockout Techniques
Homeostasis
Humans
Membrane Proteins metabolism
Mice
Mitochondrial Proteins metabolism
Phosphorylation
Protein Transport
RNA, Small Interfering genetics
RNA-Binding Proteins
Ribosomal Proteins deficiency
Ribosomal Proteins genetics
Serine metabolism
Apoptosis Regulatory Proteins metabolism
Mitochondria metabolism
Mitochondrial Dynamics
Protein Biosynthesis
Ribosomal Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 290
- Issue :
- 41
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26306039
- Full Text :
- https://doi.org/10.1074/jbc.M115.673343