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Inhibitory effect of antidepressant drugs on contact hypersensitivity reaction is connected with their suppressive effect on NKT and CD8(+) T cells but not on TCR delta T cells.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2015 Oct; Vol. 28 (2), pp. 1091-6. Date of Electronic Publication: 2015 Aug 24. - Publication Year :
- 2015
-
Abstract
- Background: Contact hypersensitivity (CHS) reaction induced by a topical application of hapten is a cell-mediated antigen-specific type of skin inflammation mediated by interaction of several subtypes of T cell subpopulations. Recently, it has been shown that antidepressant drugs inhibit CHS reaction, although the mechanism of this effect remains unknown. The aim of the present study was to investigate the effect of 2-week desipramine or fluoxetine administration on the CHS reaction induced by picryl chloride (PCL) application in B10.PL mice and in knock-out mice established on B10.PL background: TCRδ(-/-) mice lacking TCRγδ T lymphocytes; β2m(-/-) mice lacking CD8(+) T lymphocytes and CD1d(-/-) mice lacking CD1d dependent natural killer T (NKT) lymphocytes.<br />Methods: B10.PL, TCRδ(-/-), β2m(-/-) and CD1d(-/-) mice were divided into six groups: 1) vehicle-treated negative control group; 2) desipramine-treated negative control group; 3) fluoxetine-treated negative control group; 4) vehicle and PCL-treated group (positive control group); 5) desipramine and PCL-treated group; and 6) fluoxetine and PCL-treated group. CHS to PCL was tested by evaluation of ear swelling. Metabolic activity of spleen and lymph node cells were estimated by MTT test.<br />Results: The antidepressants significantly suppressed the CHS reaction in B10.PL mice: desipramine by 55% and fluoxetine by 42% compared to the positive control. This effect was even stronger in TCRδ(-/-) mice, in which fluoxetine reduced the ear swelling by 73% in comparison with the vehicle-treated positive control group. On the other hand, desipramine and fluoxetine did not inhibit CHS reaction in β2m(-/-) and CD1d(-/-) mice. Moreover, PCL increased metabolic and/or proliferative activity of splenocytes in all four strains of mice whereas the antidepressants decreased this activity of splenocytes in B10.PL, TCRδ(-/-) and CD1d(-/-) mice.<br />Conclusion: The results of the present study show that lack of CD8(+) T cells or NKT cells abolishes the immunosuppressive effect of antidepressant drugs on PCL-induced CHS reaction in mice. These results suggest that antidepressant drug-induced inhibition of CHS reaction is connected with their inhibitory effect on ability of CD8(+) T cells and NKT cells to induce and/or escalate CHS reaction. TCRγδ cells seem not to be involved in antidepressant-induced suppression of CHS.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antidepressive Agents adverse effects
Antigens, CD1d genetics
CD8-Positive T-Lymphocytes physiology
Dermatitis, Contact immunology
Desipramine adverse effects
Fluoxetine adverse effects
Immunosuppression Therapy
Mice
Mice, Inbred Strains
Mice, Knockout
Natural Killer T-Cells physiology
Picryl Chloride administration & dosage
Receptors, Antigen, T-Cell, gamma-delta genetics
Receptors, Antigen, T-Cell, gamma-delta metabolism
beta 2-Microglobulin genetics
Antidepressive Agents administration & dosage
CD8-Positive T-Lymphocytes drug effects
Dermatitis, Contact drug therapy
Desipramine administration & dosage
Fluoxetine administration & dosage
Natural Killer T-Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 28
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26315493
- Full Text :
- https://doi.org/10.1016/j.intimp.2015.08.001