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Modulation of Innate Immune Signalling by Lipid-Mediated MAVS Transmembrane Domain Oligomerization.
- Source :
-
PloS one [PLoS One] 2015 Aug 28; Vol. 10 (8), pp. e0136883. Date of Electronic Publication: 2015 Aug 28 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- RIG-I-like receptors detect viral RNA in infected cells and promote oligomerization of the outer mitochondrial membrane protein MAVS to induce innate immunity to viral infection through type I interferon production. Mitochondrial reactive oxygen species (mROS) have been shown to enhance anti-viral MAVS signalling, but the mechanisms have remained obscure. Using a biochemical oligomerization-reporter fused to the transmembrane domain of MAVS, we found that mROS inducers promoted lipid-dependent MAVS transmembrane domain oligomerization in the plane of the outer mitochondrial membrane. These events were mirrored by Sendai virus infection, which similarly induced lipid peroxidation and promoted lipid-dependent MAVS transmembrane domain oligomerization. Our observations point to a role for mROS-induced changes in lipid bilayer properties in modulating antiviral innate signalling by favouring the oligomerization of MAVS transmembrane domain in the outer-mitochondrial membrane.
- Subjects :
- Adaptor Proteins, Signal Transducing genetics
HEK293 Cells
Humans
Mitochondria metabolism
Protein Interaction Domains and Motifs
Reactive Oxygen Species metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Respirovirus Infections immunology
Sendai virus immunology
Adaptor Proteins, Signal Transducing chemistry
Adaptor Proteins, Signal Transducing metabolism
Immunity, Innate
Lipid Peroxidation
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26317833
- Full Text :
- https://doi.org/10.1371/journal.pone.0136883