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Combination of counterpropagation artificial neural networks and antioxidant activities for comprehensive evaluation of associated-extraction efficiency of various cyclodextrins in the traditional Chinese formula Xue-Zhi-Ning.
- Source :
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Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2015 Nov 10; Vol. 115, pp. 580-6. Date of Electronic Publication: 2015 Aug 12. - Publication Year :
- 2015
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Abstract
- Xue-Zhi-Ning (XZN) is a widely used traditional Chinese medicine formula to treat hyperlipidemia. Recently, cyclodextrins (CDs) have been extensively used to minimize problems relative to medicine bioavailability, such as low solubility and poor stability. The objective of this study was to determine the associated-extraction efficiency of various CDs in XZN. Three various type CDs were evaluated, including native CDs (α-CD, β-CD), hydrophilic CD derivatives (HP-β-CD and Me-β-CD), and ionic CD derivatives (SBE-β-CD and CM-β-CD). An ultra high-performance liquid chromatography (UHPLC) fingerprint was applied to determine the components in CD extracts and original aqueous extract (OAE). A counterpropagation artificial neural network (CP-ANN) was used to analyze the components in different extracts and compare the selective extraction of various CDs. Extraction efficiencies of the various CDs in terms of extracted components follow the ranking, ionic CD derivatives>hydrophilic CD derivatives>native CDs>OAE. Besides, different types of CDs have their own selective extraction and ionic CD derivatives present the strongest associated-extraction efficiency. Antioxidant potentials of various extracts were evaluated by determining the inhibition of spontaneous, H2O2-induced, CCl4-induced and Fe(2+)/ascorbic acid-induced lipid peroxidation (LPO) and analyzing the scavenging capacity for DPPH and hydroxyl radicals. The order of extraction efficiencies of the various CDs relative to antioxidant activities is as follows: SBE-β-CD>CM-β-CD>HP-β-CD>Me-β-CD>β-CD>α-CD. It can be demonstrated that all of the CDs studied increase the extraction efficiency and that ionic CD derivatives (SBE-β-CD and CM-β-CD) present the highest extraction capability in terms of amount extracted and antioxidant activities of extracts.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Antioxidants chemistry
Antioxidants pharmacology
Biphenyl Compounds chemistry
Chemistry, Pharmaceutical
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal chemistry
Drugs, Chinese Herbal pharmacology
Hydroxyl Radical chemistry
Lipid Peroxidation
Picrates chemistry
Antioxidants isolation & purification
Cyclodextrins chemistry
Drugs, Chinese Herbal isolation & purification
Neural Networks, Computer
Technology, Pharmaceutical methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-264X
- Volume :
- 115
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical and biomedical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 26322951
- Full Text :
- https://doi.org/10.1016/j.jpba.2015.08.006