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Circulating Histone Levels Reflect Disease Severity in Animal Models of Acute Pancreatitis.
- Source :
-
Pancreas [Pancreas] 2015 Oct; Vol. 44 (7), pp. 1089-95. - Publication Year :
- 2015
-
Abstract
- Objectives: Extracellular histones are rapidly cleared by the liver and rarely detectable in the circulation unless there is extensive cell death, as in severe trauma and sepsis. This study investigated whether circulating histones are elevated in experimental acute pancreatitis models and correlate to disease severity.<br />Methods: Acute pancreatitis was induced in mice by: (1) 4 or (2) 12 intraperitoneal injections of cerulein (50 μg/kg) at 1 hour apart; (3) retrograde infusion of 3.5% sodium taurocholate into the biliopancreatic duct. Mice were sacrificed at various time points to collect blood and tissues. Severity of pancreatitis was assessed by biochemical markers and histopathology. Circulating histones were determined by Western blotting.<br />Results: Four cerulein injections induced edematous pancreatitis, whereas 12 cerulein injections and ductal taurocholate infusion caused necrotizing pancreatitis. Circulating histones were barely detectable in the blood of animals with edematous pancreatitis but significantly increased in necrotizing pancreatitis. The levels of circulating histones were strongly correlated to histopathological scores of necrosis of the pancreas.<br />Conclusions: Circulating histones increased significantly in necrotizing pancreatitis due to extensive pancreatic acinar cell death. Levels of circulating histones may have translational potential as a biomarker of disease severity.
- Subjects :
- Acute Disease
Analysis of Variance
Animals
Biomarkers blood
Blotting, Western
Ceruletide
Humans
Male
Mice, Inbred C57BL
Pancreatitis chemically induced
Pancreatitis pathology
Pancreatitis, Acute Necrotizing chemically induced
Pancreatitis, Acute Necrotizing pathology
Severity of Illness Index
Taurocholic Acid
Disease Models, Animal
Histones blood
Pancreatitis blood
Pancreatitis, Acute Necrotizing blood
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4828
- Volume :
- 44
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Pancreas
- Publication Type :
- Academic Journal
- Accession number :
- 26335015
- Full Text :
- https://doi.org/10.1097/MPA.0000000000000416