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A short-type peptidoglycan recognition protein from tongue sole (Cynoglossus semilaevis) promotes phagocytosis and defense against bacterial infection.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2015 Nov; Vol. 47 (1), pp. 313-20. Date of Electronic Publication: 2015 Sep 11. - Publication Year :
- 2015
-
Abstract
- Peptidoglycan recognition proteins (PGRPs) are members of the innate immune system that interact with bacteria by binding to bacterial peptidoglycan. In this study, we examined the expression and function of a short type of PGRP, CsPGRP-SC2, from tongue sole (Cynoglossus semilaevis). CsPGRP-SC2 contains 164 amino acid residues and shares 54.5%-65.3% overall sequence identities with other teleost PGRPs. CsPGRP-SC2 possesses an amidase domain with a conserved zinc binding site. CsPGRP-SC2 expression occurred in multiple tissues and was upregulated by bacterial and viral infection. Purified recombinant CsPGRP-SC2 (rCsPGRP-SC2) was able to bind and agglutinate Gram-positive bacteria in a Zn(2+)-dependent manner. rCsPGRP-SC2 enhanced the uptake of the bound bacteria by host phagocytes and reduced bacterial dissemination and colonization in host tissues. These results indicate that CsPGRP-SC2 is an innate immune factor that participates in host defense against bacterial infection.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Carrier Proteins chemistry
Carrier Proteins metabolism
DNA Virus Infections genetics
DNA Virus Infections immunology
DNA Virus Infections virology
Fish Diseases immunology
Fish Diseases microbiology
Fish Diseases virology
Fish Proteins chemistry
Fish Proteins metabolism
Iridoviridae physiology
Phagocytosis
Real-Time Polymerase Chain Reaction veterinary
Sequence Alignment veterinary
Streptococcal Infections genetics
Streptococcal Infections immunology
Streptococcal Infections microbiology
Streptococcus physiology
Carrier Proteins genetics
DNA Virus Infections veterinary
Fish Diseases genetics
Fish Proteins genetics
Flatfishes
Immunity, Innate
Streptococcal Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 26364742
- Full Text :
- https://doi.org/10.1016/j.fsi.2015.09.021