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[Determination of trichothecenes in cereals].

Authors :
Czerwiecki L
Giryn H
Source :
Roczniki Panstwowego Zakladu Higieny [Rocz Panstw Zakl Hig] 1989; Vol. 40 (4-6), pp. 284-90.
Publication Year :
1989

Abstract

A simple method is described of identification and determination of deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS) and T-2 toxin in cereals. Chloroform-ethanol extracts were purified in columns filled with active charcoal, aluminium oxide and celite and were analysed by the method of thin-layer chromatography. The results were evaluated exposing fluorescent trichothecene derivatives to ultraviolet light, after they had been obtained on a chromatographic plate with aluminium chloride and sulphuric acid. The detectability of the method for various toxins was: DON--37, NIV--100, DAS--50, and T-2 toxin--100 mcg/kg.

Details

Language :
Polish
ISSN :
0035-7715
Volume :
40
Issue :
4-6
Database :
MEDLINE
Journal :
Roczniki Panstwowego Zakladu Higieny
Publication Type :
Academic Journal
Accession number :
2637481