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Functional characterization of DnSIZ1, a SIZ/PIAS-type SUMO E3 ligase from Dendrobium.
- Source :
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BMC plant biology [BMC Plant Biol] 2015 Sep 17; Vol. 15, pp. 225. Date of Electronic Publication: 2015 Sep 17. - Publication Year :
- 2015
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Abstract
- Background: SUMOylation is an important post-translational modification of eukaryotic proteins that involves the reversible conjugation of a small ubiquitin-related modifier (SUMO) polypeptide to its specific protein substrates, thereby regulating numerous complex cellular processes. The PIAS (protein inhibitor of activated signal transducers and activators of transcription [STAT]) and SIZ (scaffold attachment factor A/B/acinus/PIAS [SAP] and MIZ) proteins are SUMO E3 ligases that modulate SUMO conjugation. The characteristic features and SUMOylation mechanisms of SIZ1 protein in monocotyledon are poorly understood. Here, we examined the functions of a homolog of Arabidopsis SIZ1, a functional SIZ/PIAS-type SUMO E3 ligase from Dendrobium.<br />Results: In Dendrobium, the predicted DnSIZ1 protein has domains that are highly conserved among SIZ/PIAS-type proteins. DnSIZ1 is widely expressed in Dendrobium organs and has a up-regulated trend by treatment with cold, high temperature and wounding. The DnSIZ1 protein localizes to the nucleus and shows SUMO E3 ligase activity when expressed in an Escherichia coli reconstitution system. Moreover, ectopic expression of DnSIZ1 in the Arabidopsis siz1-2 mutant partially complements several phenotypes and results in enhanced levels of SUMO conjugates in plants exposed to heat shock conditions. We observed that DnSIZ1 acts as a negative regulator of flowering transition which may be via a vernalization-induced pathway. In addition, ABA-hypersensitivity of siz1-2 seed germination can be partially suppressed by DnSIZ1.<br />Conclusions: Our results suggest that DnSIZ1 is a functional homolog of the Arabidopsis SIZ1 with SUMO E3 ligase activity and may play an important role in the regulation of Dendrobium stress responses, flowering and development.
- Subjects :
- Arabidopsis genetics
Arabidopsis metabolism
Dendrobium metabolism
Heat-Shock Response
Molecular Sequence Data
Phylogeny
Plant Proteins chemistry
Plant Proteins metabolism
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Sequence Alignment
Sequence Analysis, Protein
Sumoylation
Ubiquitin-Protein Ligases chemistry
Ubiquitin-Protein Ligases metabolism
Dendrobium genetics
Gene Expression Regulation, Plant
Plant Proteins genetics
Ubiquitin-Protein Ligases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2229
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- BMC plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 26376625
- Full Text :
- https://doi.org/10.1186/s12870-015-0613-3