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Inhibition of Hepatocyte Apoptosis: An Important Mechanism of Corn Peptides Attenuating Liver Injury Induced by Ethanol.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2015 Sep 11; Vol. 16 (9), pp. 22062-80. Date of Electronic Publication: 2015 Sep 11. - Publication Year :
- 2015
-
Abstract
- In this study, the effects of mixed corn peptides and synthetic pentapeptide (QLLPF) on hepatocyte apoptosis induced by ethanol were investigated in vivo. QLLPF, was previously characterized from corn protein hydrolysis, which had been shown to exert good facilitating alcohol metabolism activity. Mice were pre-treated with the mixed corn peptides and the pentapeptide for 1 week and then treated with ethanol. After treatment of three weeks, the biochemical indices and the key ethanol metabolizing enzymes, the serum TNF-α, liver TGF-β1 concentrations and the protein expressions related to apoptosis were determined. We found that the Bcl-2, Bax and cytochrome c expressions in the intrinsic pathway and the Fas, FasL and NF-κB expressions in the extrinsic pathway together with higher TNF-α and TGF-β1 concentrations were reversed compared with the model group by both the mixed corn peptides and the pentapeptide. The activation of caspase3 was also suppressed. Additionally, apoptosis was further confirmed with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and the TUNEL assay demonstrated peptides suppressed hepatocyte apoptosis. Our results suggest that apoptosis induced by ethanol is alleviated in response to the treatment of corn peptides, potentially due to reversing the related protein expression.
- Subjects :
- Animals
Body Weight drug effects
Caspase 3 genetics
Caspase 3 metabolism
Chemical and Drug Induced Liver Injury drug therapy
Chemical and Drug Induced Liver Injury genetics
Chemical and Drug Induced Liver Injury metabolism
Chemical and Drug Induced Liver Injury pathology
Cytokines metabolism
Disease Models, Animal
Ethanol adverse effects
Ethanol metabolism
Gene Expression genetics
Liver drug effects
Liver metabolism
Liver pathology
Liver Function Tests
Male
Metabolic Networks and Pathways drug effects
Mice
Oxidation-Reduction drug effects
Peptides chemistry
Plant Extracts chemistry
Signal Transduction drug effects
Apoptosis drug effects
Hepatocytes drug effects
Hepatocytes metabolism
Peptides pharmacology
Plant Extracts pharmacology
Zea mays chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 16
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 26378531
- Full Text :
- https://doi.org/10.3390/ijms160922062