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Intracellular distribution of fluorescent copper and zinc bis(thiosemicarbazonato) complexes measured with fluorescence lifetime spectroscopy.
- Source :
-
Inorganic chemistry [Inorg Chem] 2015 Oct 05; Vol. 54 (19), pp. 9556-67. Date of Electronic Publication: 2015 Sep 23. - Publication Year :
- 2015
-
Abstract
- The intracellular distribution of fluorescently labeled copper and zinc bis(thiosemicarbazonato) complexes was investigated in M17 neuroblastoma cells and primary cortical neurons with a view to providing insights into the neuroprotective activity of a copper bis(thiosemicarbazonato) complex known as Cu(II)(atsm). Time-resolved fluorescence measurements allowed the identification of the Cu(II) and Zn(II) complexes as well as the free ligand inside the cells by virtue of the distinct fluorescence lifetime of each species. Confocal fluorescent microscopy of cells treated with the fluorescent copper(II)bis(thiosemicarbazonato) complex revealed significant fluorescence associated with cytoplasmic puncta that were identified to be lysosomes in primary cortical neurons and both lipid droplets and lysosomes in M17 neuroblastoma cells. Fluorescence lifetime imaging microscopy confirmed that the fluorescence signal emanating from the lipid droplets could be attributed to the copper(II) complex but also that some degree of loss of the metal ion led to diffuse cytosolic fluorescence that could be attributed to the metal-free ligand. The accumulation of the copper(II) complex in lipid droplets could be relevant to the neuroprotective activity of Cu(II)(atsm) in models of amyotrophic lateral sclerosis and Parkinson's disease.
- Subjects :
- Cell Line, Tumor
Coordination Complexes chemical synthesis
Coordination Complexes chemistry
Humans
Models, Molecular
Molecular Structure
Spectrometry, Fluorescence
Time Factors
Tissue Distribution
Coordination Complexes pharmacokinetics
Copper chemistry
Fluorescence
Thiosemicarbazones chemistry
Zinc chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-510X
- Volume :
- 54
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Inorganic chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26397162
- Full Text :
- https://doi.org/10.1021/acs.inorgchem.5b01599