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Imaging and Antitumoral Effect of a Cyclo-oxygenase 2-specific Replicative Adenovirus for Small Metastatic Gastric Cancer Lesions.
- Source :
-
Anticancer research [Anticancer Res] 2015 Oct; Vol. 35 (10), pp. 5201-10. - Publication Year :
- 2015
-
Abstract
- Background: Long-term outcomes of patients with peritoneal dissemination of gastric cancer remain unsatisfactory despite advances in treatment modalities. Internal luminescence conditionally replicative adenovirus (CRAd) presents a novel approach for cancer treatment and imaging.<br />Materials and Methods: 3CL is a modified cyclooxygenase-2 (COX2) promoter-driven CRAd which contains the luciferase expression gene for bioluminescence imaging. The visualizing and therapeutic effect of 3CL was evaluated in a mouse model of peritoneal dissemination.<br />Results: Intraperitoneal injection of 3CL achieved the shrinkage and reduction of lesions of peritoneal dissemination. Six model mice treated with 3CL had a significantly longer mean survival time than 6 mock-treated mice (85.7 versus 34.3 days, p=0.0005). By whole-body bioluminescent imaging, the sensitivity and specificity of peritoneal dissemination detection through macroscopic inspection were 58.1% and 83.2%, respectively, whereas 3CL viral imaging modality yielded corresponding values of 78.8% and 99.3%. Peritoneal lesions detected by imaging histologically contained cancer cells and necrotic tissue, which originated from viral oncolytic effects.<br />Conclusion: Cox2 CRAds with 5/3 chimeric-fiber modification, therefore, appear to be a promising imaging and therapeutic tools for peritoneal dissemination of gastric cancer.<br /> (Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Subjects :
- Animals
Cell Line, Tumor
Cyclooxygenase 2 genetics
Dependovirus genetics
Female
Genetic Vectors administration & dosage
Humans
Luciferases genetics
Mice
Neoplasm Transplantation
Peritoneal Neoplasms secondary
Recombinant Proteins genetics
Recombinant Proteins metabolism
Sensitivity and Specificity
Survival Analysis
Treatment Outcome
Xenograft Model Antitumor Assays
Cyclooxygenase 2 metabolism
Dependovirus physiology
Diagnostic Imaging methods
Luciferases metabolism
Peritoneal Neoplasms therapy
Peritoneal Neoplasms virology
Stomach Neoplasms therapy
Stomach Neoplasms virology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 35
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 26408678