Back to Search
Start Over
Brevibacterium metallicus sp. nov., an endophytic bacterium isolated from roots of Prosopis laegivata grown at the edge of a mine tailing in Mexico.
- Source :
-
Archives of microbiology [Arch Microbiol] 2015 Dec; Vol. 197 (10), pp. 1151-8. Date of Electronic Publication: 2015 Oct 01. - Publication Year :
- 2015
-
Abstract
- A Gram-positive, aerobic, nonmotile strain, NM2E3(T) was identified as Brevibacterium based on the 16S rRNA gene sequence analysis and had the highest similarities to Brevibacterium jeotgali SJ5-8(T) (97.3 %). This novel bacterium was isolated from root tissue of Prosopis laegivata grown at the edge of a mine tailing in San Luis Potosí, Mexico. Its cells were non-spore-forming rods, showing catalase and oxidase activities and were able to grow in LB medium added with 40 mM Cu(2+), 72 mM As(5+) and various other toxic elements. Anteiso-C15:0 (41.6 %), anteiso-C17:0 (30 %) and iso-C15:0 (9.5 %) were the major fatty acids. MK-8(H2) (88.4 %) and MK-7(H2) (11.6 %) were the major menaquinones. The DNA G + C content of the strain NM2E3(T) was 70.8 mol % (Tm). DNA-DNA hybridization showed that the strain NM2E3(T) had 39.8, 21.7 and 20.3 % relatedness with B. yomogidense JCM 17779(T), B. jeotgali JCM 18571(T) and B. salitolerans TRM 45(T), respectively. Based on the phenotypic and genotypic analyses, the strain NM2E3(T) (=CCBAU 101093(T) = HAMBI 3627(T) = LMG 8673(T)) is reported as a novel species of the genus Brevibacterium, for which the name Brevibacterium metallicus sp. nov., is proposed.
- Subjects :
- Bacterial Typing Techniques
Base Composition genetics
Brevibacterium classification
Brevibacterium genetics
DNA, Bacterial genetics
Fatty Acids analysis
Mexico
Mining
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Vitamin K 2 analysis
Brevibacterium isolation & purification
Brevibacterium metabolism
Metals, Heavy metabolism
Plant Roots microbiology
Prosopis microbiology
Symbiosis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-072X
- Volume :
- 197
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Archives of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26429721
- Full Text :
- https://doi.org/10.1007/s00203-015-1156-6