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Astragaloside IV attenuates apoptosis of hypertrophic cardiomyocyte through inhibiting oxidative stress and calpain-1 activation.
- Source :
-
Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2015 Nov; Vol. 40 (3), pp. 764-73. Date of Electronic Publication: 2015 Sep 16. - Publication Year :
- 2015
-
Abstract
- Calpain-1 activation and oxidative stress are two critical factors contributing to apoptosis of hypertrophic cardiomyocyte. Astragaloside IV (ASIV) exhibits protective effect against various heart diseases. The present study was designed to investigate whether the inhibitory effect of ASIV on isoproterenol (ISO)-induced apoptosis of hypertrophic cardiomyocyte was associated with the anti-oxidation and calpain-1 inhibition. Hypertrophy, apoptosis, mitochondrial oxidative stress and calpain-1 expression were measured in the heart tissue of Sprague-Dawley (SD) rats and H9C2 cells treated with ISO alone or combination with ASIV. The results showed that ASIV attenuated apoptotic rate, increased Bcl-2 expression, decreased Bax expression, ameliorated the integrity of mitochondrial structure and improved mitochondrial membrane potential (MMP). Moreover, ASIV combination reduced both calpain-1 protein expression and calpain activity, down-regulated mitochondrial NOX4 (mito-NOX4) expression, increased activity of mitochondrial superoxide dismutase (mito-SOD) and mitochondrial catalase (mito-CAT) compared to ISO treated alone. The results suggested that ASIV exerted anti-apoptosis effect on ISO-induced hypertrophic cardiomyocyte by attenuating oxidative stress and calpain-1 activation.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antioxidants pharmacology
Cardiomegaly chemically induced
Cardiomegaly metabolism
Cardiotonic Agents pharmacology
Cell Line
Gene Expression Regulation drug effects
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Myocytes, Cardiac cytology
Myocytes, Cardiac drug effects
Myocytes, Cardiac pathology
Oxidative Stress drug effects
Rats
Rats, Sprague-Dawley
Saponins pharmacology
Triterpenes pharmacology
2-Propanol toxicity
Antioxidants administration & dosage
Apoptosis drug effects
Calpain metabolism
Cardiomegaly prevention & control
Saponins administration & dosage
Triterpenes administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7077
- Volume :
- 40
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Environmental toxicology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26433482
- Full Text :
- https://doi.org/10.1016/j.etap.2015.09.007