[Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media].

Authors :: Zhang HY
Xu SJ
Tang XX
Niu JJ
Guo XJ
Gao CR
Source :: Fa yi xue za zhi [Fa Yi Xue Za Zhi] 2015 Jun; Vol. 31 (3), pp. 188-90.
Publication Year :: 2015
Abstract: Objective: To investigate the diagnostic significance of basophil activation test (BAT) in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 (MC-CPA3), and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to β-lactam drugs and non -ionic contrast media.<br />Methods: The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA.<br />Results: The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs were significantly higher than that in normal immune group (P < 0.05).<br />Conclusion: There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.