Back to Search
Start Over
Establishment and evaluation of a stable steroidogenic goat Leydig cell line.
- Source :
-
Animal science journal = Nihon chikusan Gakkaiho [Anim Sci J] 2016 Apr; Vol. 87 (4), pp. 492-502. Date of Electronic Publication: 2015 Oct 14. - Publication Year :
- 2016
-
Abstract
- Leydig cells play a key role in synthesizing androgen and regulating spermatogenesis. The dysfunction of Leydig cells may lead to various male diseases. Although primary Leydig cell cultures have been used, their finite lifespan hinders the assessment of long-term effects. In the present study, primary goat Leydig cells (GLCs) were immortalized via the transfection of a plasmid containing the human telomerase reverse transcriptase (hTERT) gene. The expressions of hTERT and telomerase activity were evaluated in transduced GLCs (hTERT-GLCs). These cells steadily expressed the hTERT gene and exhibited longer telomere lengths at passage 55 that were similar to those of HeLa cells. The hTERT-GLCs at passages 30 and 50 expressed genes that encoded key proteins, enzymes and receptors that are inherent to normal Leydig cells, for example, steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD) and LH-receptor (LH-R). Additionally, the immortalized goat Leydig cells secreted detectable quantities of testosterone in response to hCG stimulation. Furthermore, this cell line appeared to proliferate more quickly than the control cells, although no neoplastic transformation occurred in vitro. We concluded that the GLCs immortalized with hTERT retained their original characteristics and might provide a useful model for the study of Leydig cell function.<br /> (© 2015 Japanese Society of Animal Science.)
- Subjects :
- 17-Hydroxysteroid Dehydrogenases metabolism
Animals
Cell Line
Cell Proliferation
Cholesterol Side-Chain Cleavage Enzyme metabolism
Chorionic Gonadotropin physiology
Cytochrome P-450 Enzyme System metabolism
Goats
Male
Phosphoproteins metabolism
Receptors, LH metabolism
Spermatogenesis
Telomerase genetics
Telomerase metabolism
Telomere Homeostasis
Leydig Cells cytology
Leydig Cells enzymology
Leydig Cells metabolism
Leydig Cells physiology
Testosterone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1740-0929
- Volume :
- 87
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Animal science journal = Nihon chikusan Gakkaiho
- Publication Type :
- Academic Journal
- Accession number :
- 26462462
- Full Text :
- https://doi.org/10.1111/asj.12454