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Down-regulation of neutrophil gelatinase-associated lipocalin in head and neck squamous cell carcinoma correlated with tumorigenesis, not with metastasis.
- Source :
-
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2015 Aug 01; Vol. 8 (8), pp. 8857-68. Date of Electronic Publication: 2015 Aug 01 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- To examine the significance of the Neutrophil gelatinase-associated lipocalin (NGAL) in diagnosing head and neck squamous cell carcinoma (HNSCC) and predicting regional metastasis. We first used GEO dataset to analyze the NGAL gene expression in HNSCC. Then, we summarized the characteristics of patients retrospectively selected in clinic. Expression of NGAL protein in human HNSCC tumor, lymph node and normal samples were analyzed using immunohistochemistry. Next, we further investigated the NGAL expression in a tissue microassay to analyze the relationship between NGAL protein expression and TNM stage. Finally, we tested the NGAL protein expression in head and neck cancer cell lines. Analysis of GEO dataset concluded that NGAL gene expression in HNSCC was lower than that in normal tissue (P<0.01). There was no statistically significant difference of NGAL gene expression between T-stage and N-stage (P>0.05). NGAL protein expression in tumor was lower than that in normal tissue (P<0.01). There was no statistically significant difference of NGAL protein expression between metastasis group and non-metastasis group (P>0.05). Expression of NGAL protein was not correlated with TNM stage of HNSCC. Aggressive HNSCC cell lines have lower NGAL protein expression. Our data demonstrated that the expression of NGAL protein was correlated with tumorigenesis of HNSCC, but not with regional metastasis. It may serve as a novel biomarker for prognostic evaluation of patients with HNSCC.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor analysis
Blotting, Western
Carcinogenesis genetics
Carcinoma, Squamous Cell genetics
Down-Regulation
Female
Head and Neck Neoplasms genetics
Humans
Immunohistochemistry
Lipocalin-2
Male
Middle Aged
Neoplasm Invasiveness genetics
Oligonucleotide Array Sequence Analysis
Retrospective Studies
Squamous Cell Carcinoma of Head and Neck
Tissue Array Analysis
Transcriptome
Young Adult
Acute-Phase Proteins biosynthesis
Carcinogenesis pathology
Carcinoma, Squamous Cell pathology
Head and Neck Neoplasms pathology
Lipocalins biosynthesis
Neoplasm Invasiveness pathology
Proto-Oncogene Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1936-2625
- Volume :
- 8
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- International journal of clinical and experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 26464627