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A cotton Raf-like MAP3K gene, GhMAP3K40, mediates reduced tolerance to biotic and abiotic stress in Nicotiana benthamiana by negatively regulating growth and development.
- Source :
-
Plant science : an international journal of experimental plant biology [Plant Sci] 2015 Nov; Vol. 240, pp. 10-24. Date of Electronic Publication: 2015 Aug 24. - Publication Year :
- 2015
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Abstract
- Mitogen-activated protein kinase (MAPK) cascades mediate various responses in plants. As the top component, MAP3Ks deserve more attention; however, little is known about the role of MAP3Ks, especially in cotton, a worldwide economic crop. In this study, a gene encoding a putative Raf-like MAP3K, GhMAP3K40, was isolated. GhMAP3K40 expression was induced by stress and multiple signal molecules. The plants overexpressing GhMAP3K40 had an enhanced tolerance to drought and salt stress at the germination stage. However, at the seedling stage, the transgenic plants suffered more severe damage after drought, exposure to pathogens and oxidative stress. The defence-related genes and the antioxidant system were activated in transgenic palnts, suggesting that GhMAP3K40 positively regulate the defence response. The transgenic plants were less able to prevent pathogenic invasion, which was due to defects in the cell structure of the leaves. The root system of the control plants were stronger compared with the transgenic plants. These results indicated a negative role of GhMAP3K40 in growth and development and GhMAP3K40 possibly caused the defects by down-regulating the lignin biosynthesis. Overall, these results suggest that GhMAP3K40 may positively regulate defence response but cause reduced tolerance to biotic and abiotic stress by negatively regulating growth and development.<br /> (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Ectopic Gene Expression
Gossypium metabolism
Mitogen-Activated Protein Kinases chemistry
Phylogeny
Plant Proteins chemistry
Plant Proteins genetics
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Sequence Alignment
Stress, Physiological genetics
Nicotiana genetics
Nicotiana growth & development
Gene Expression Regulation, Plant
Gossypium genetics
Mitogen-Activated Protein Kinases genetics
Mitogen-Activated Protein Kinases metabolism
Plant Proteins metabolism
Nicotiana physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2259
- Volume :
- 240
- Database :
- MEDLINE
- Journal :
- Plant science : an international journal of experimental plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 26475184
- Full Text :
- https://doi.org/10.1016/j.plantsci.2015.08.012