Potentiometric chemosensor for neopterin, a cancer biomarker, using an electrochemically synthesized molecularly imprinted polymer as the recognition unit.

Authors :: Sharma PS
Wojnarowicz A
Sosnowska M
Benincori T
Noworyta K
D'Souza F
Kutner W
Source :: Biosensors & bioelectronics [Biosens Bioelectron] 2016 Mar 15; Vol. 77, pp. 565-72. Date of Electronic Publication: 2015 Nov 14.
Publication Year :: 2016
Abstract: With an established procedure of molecular imprinting, a synthetic polymer receptor for the neopterin cancer biomarker was devised and used as a recognition unit of a potentiometric chemosensor. For that, bis-bithiophene derivatized with cytosine and bithiophene derivatized with boronic acid were used as functional monomers. The open-circuit potential (OCP) based transduction under flow-injection analysis conditions (FIA) determined neopterin in the concentration range of 0.15-2.5mM with the 22 µM limit of detection (LOD) and 7.01(±0.15) mVmM(-1) sensitivity indicating its potential suitability in clinical analysis applications. The molecularly imprinted polymer (MIP) film showed an appreciable apparent imprinting factor of ~6. The chemosensor successfully discriminated the interferences including the 6-biopterin and pterin structural analogs of neopterin as well as glucose and creatinine. Moreover, it determined neopterin in synthetic serum samples.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Electroplating methods
Equipment Design
Equipment Failure Analysis
Reproducibility of Results
Sensitivity and Specificity
Surface Properties
Biomarkers, Tumor analysis
Conductometry instrumentation
Molecular Imprinting methods
Neopterin blood
Polymers chemistry
Potentiometry instrumentation

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