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Phenotypic, Morphological and Adhesive Differences of Human Hematopoietic Progenitor Cells Cultured on Murine versus Human Mesenchymal Stromal Cells.
- Source :
-
Scientific reports [Sci Rep] 2015 Oct 26; Vol. 5, pp. 15680. Date of Electronic Publication: 2015 Oct 26. - Publication Year :
- 2015
-
Abstract
- Xenogenic transplantation models have been developed to study human hematopoiesis in immunocompromised murine recipients. They still have limitations and therefore it is important to delineate all players within the bone marrow that could account for species-specific differences. Here, we evaluated the proliferative capacity, morphological and physical characteristics of human CD34(+) hematopoietic stem and progenitor cells (HSPCs) after co-culture on murine or human bone marrow-derived mesenchymal stromal cells (MSCs). After seven days, human CD34(+)CD133(-) HSPCs expanded to similar extents on both feeder layers while cellular subsets comprising primitive CD34(+)CD133(+) and CD133(+)CD34(-) phenotypes are reduced fivefold on murine MSCs. The number of migrating HSPCs was also reduced on murine cells suggesting that MSC adhesion influences cellular polarization of HSPC. We used atomic force microscopy-based single-cell force spectroscopy to quantify their adhesive interactions. We found threefold higher detachment forces of human HSPCs from murine MSCs compared to human ones. This difference is related to the N-cadherin expression level on murine MSCs since its knockdown abolished their differential adhesion properties with human HSPCs. Our observations highlight phenotypic, morphological and adhesive differences of human HSPCs when cultured on murine or human MSCs, which raise some caution in data interpretation when xenogenic transplantation models are used.
- Subjects :
- AC133 Antigen
Animals
Antigens, CD metabolism
Antigens, CD34 metabolism
Cadherins antagonists & inhibitors
Cadherins genetics
Cadherins metabolism
Cell Differentiation
Cell Movement
Cell Polarity
Cells, Cultured
Coculture Techniques
Glycoproteins metabolism
Hematopoiesis
Hematopoietic Stem Cells metabolism
Humans
Mesenchymal Stem Cells metabolism
Mice
Mice, Inbred C57BL
Microscopy, Atomic Force
Peptides metabolism
Phenotype
RNA Interference
Transport Vesicles metabolism
Cell Adhesion
Hematopoietic Stem Cells cytology
Mesenchymal Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26498381
- Full Text :
- https://doi.org/10.1038/srep15680