Back to Search
Start Over
Design and evaluation of an intravesical delivery system for superficial bladder cancer: preparation of gemcitabine HCl-loaded chitosan-thioglycolic acid nanoparticles and comparison of chitosan/poloxamer gels as carriers.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2015 Oct 14; Vol. 10, pp. 6493-507. Date of Electronic Publication: 2015 Oct 14 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- This study aimed to develop an intravesical delivery system of gemcitabine HCl for superficial bladder cancer in order to provide a controlled release profile, to prolong the residence time, and to avoid drug elimination via urination. For this aim, bioadhesive nanoparticles were prepared with thiolated chitosan (chitosan-thioglycolic acid conjugate) and were dispersed in bioadhesive chitosan gel or in an in situ gelling poloxamer formulation in order to improve intravesical residence time. In addition, nanoparticle-loaded gels were diluted with artificial urine to mimic in vivo conditions in the bladder and were characterized regarding changes in gel structure. The obtained results showed that chitosanthioglycolic acid nanoparticles with a mean diameter of 174.5±3.762 nm and zeta potential of 32.100±0.575 mV were successfully developed via ionotropic gelation and that the encapsulation efficiency of gemcitabine HCl was nearly 20%. In vitro/ex vivo characterization studies demonstrated that both nanoparticles and nanoparticle-loaded chitosan and poloxamer gels might be alternative carriers for intravesical administration of gemcitabine HCl, prolonging its residence time in the bladder and hence improving treatment efficacy. However, when the gel formulations were diluted with artificial urine, poloxamer gels lost their in situ gelling properties at body temperature, which is in conflict with the aimed formulation property. Therefore, 2% chitosan gel formulation was found to be a more promising carrier system for intravesical administration of nanoparticles.
- Subjects :
- Administration, Intravesical
Antimetabolites, Antineoplastic administration & dosage
Antimetabolites, Antineoplastic pharmacology
Chemistry, Pharmaceutical
Deoxycytidine administration & dosage
Deoxycytidine pharmacology
Gels chemistry
Humans
Nanoparticles chemistry
Poloxamer chemistry
Thioglycolates chemistry
Tumor Cells, Cultured
Gemcitabine
Chitosan chemistry
Deoxycytidine analogs & derivatives
Drug Carriers chemistry
Drug Delivery Systems
Drug Design
Nanoparticles administration & dosage
Urinary Bladder Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 26508855
- Full Text :
- https://doi.org/10.2147/IJN.S93750