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Molecular characterization, tissue distribution, and immune reaction expression of karyopherins in the domestic silkworm (Bombyx mori).
- Source :
-
Genetics and molecular research : GMR [Genet Mol Res] 2015 Oct 26; Vol. 14 (4), pp. 13049-65. Date of Electronic Publication: 2015 Oct 26. - Publication Year :
- 2015
-
Abstract
- Karyopherins, including alpha and beta types, are transport proteins in the eukaryotic cell that carry cargoes across nuclear pore complexes into or out of the nucleus. In this study, full open reading frames of one beta and three alpha types of karyopherin were cloned from cDNA of the domestic silkworm (Bombyx mori). The one beta and three alpha types' open reading frames were 2661, 1563, 1515, and 1551 base pairs long, respectively, and coded 886, 520, 504, and 516 amino acids, respectively. The alphas all had one importin-beta-binding (IBB) domain, and eight, four, or seven armadillo/beta-catenin-like repeats. The beta had 19 HEAT repeat domains, which constructed one importin-beta-N-terminal domain and one IBB domain. The recombinant proteins were expressed in Escherichia coli cells. The molecular weight of the beta type was approximately 100 kDa, and the alphas weighed approximately 60 kDa. Phylogenic tree construction revealed that the alphas could be classified into three known karyopherin-alpha subfamilies. We detected mRNA of the four karyopherins in normal 3rd day of 5th instar larvae, and in larvae injected with Gram-positive bacteria, Gram-negative bacteria, viruses, and fungi using real-time fluorescence quantitative reverse transcriptase-polymerase chain reaction, and found that the four karyopherins were widely distributed, but their expression levels were related to tissues type, the microbe injected, and the time point.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Bombyx classification
Cloning, Molecular
Karyopherins chemistry
Karyopherins metabolism
Molecular Sequence Data
Open Reading Frames
Organ Specificity genetics
Phylogeny
RNA, Messenger genetics
Recombinant Proteins genetics
Recombinant Proteins metabolism
Sequence Alignment
Sequence Analysis, DNA
Bombyx genetics
Bombyx immunology
Gene Expression
Immunity
Karyopherins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1676-5680
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Genetics and molecular research : GMR
- Publication Type :
- Academic Journal
- Accession number :
- 26535618
- Full Text :
- https://doi.org/10.4238/2015.October.26.1