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Phenotypic and Functional Properties of Human Steady State CD14+ and CD1a+ Antigen Presenting Cells and Epidermal Langerhans Cells.
- Source :
-
PloS one [PLoS One] 2015 Nov 25; Vol. 10 (11), pp. e0143519. Date of Electronic Publication: 2015 Nov 25 (Print Publication: 2015). - Publication Year :
- 2015
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Abstract
- Cutaneous antigen presenting cells (APCs) are critical for the induction and regulation of skin immune responses. The human skin contains phenotypically and functionally distinct APCs subsets that are present at two separated locations. While CD1ahigh LCs form a dense network in the epidermis, the CD14+ and CD1a+ APCs reside in the dermal compartment. A better understanding of the biology of human skin APC subsets is necessary for the improvement of vaccine strategies that use the skin as administration route. In particular, progress in the characterization of uptake and activatory receptors will certainly improve APC-targeting strategies in vaccination. Here we performed a detailed analysis of the expression and function of glycan-binding and pattern-recognition receptors in skin APC subsets. The results demonstrate that under steady state conditions human CD1a+ dermal dendritic cells (DCs) were phenotypically most mature as measured by the expression of CD83 and CD86, whereas the CD14+ cells showed a higher expression of the CLRs DC-SIGN, mannose receptor and DCIR and had potent antigen uptake capacity. Furthermore, steady state LCs showed superior antigen cross-presentation as compared to the dermal APC subsets. Our results also demonstrate that the TLR3 ligand polyribosinic-polyribocytidylic acid (pI:C) was the most potent stimulator of cytokine production by both LCs and dDCs. These studies warrant further exploration of human CD1a+ dDCs and LCs as target cells for cancer vaccination to induce anti-tumor immune responses.
- Subjects :
- Antigen Presentation immunology
Antigen-Presenting Cells immunology
Biomarkers
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes metabolism
Calcitonin Receptor-Like Protein genetics
Calcitonin Receptor-Like Protein metabolism
Cluster Analysis
Cross-Priming immunology
Cytokines genetics
Cytokines metabolism
Dendritic Cells immunology
Dendritic Cells metabolism
Gene Expression
Gene Expression Profiling
Humans
Immunophenotyping
Inflammation Mediators
Langerhans Cells immunology
Ligands
Toll-Like Receptors genetics
Toll-Like Receptors metabolism
Antigen-Presenting Cells metabolism
Antigens, CD1 metabolism
Epidermal Cells
Epidermis physiology
Langerhans Cells metabolism
Lipopolysaccharide Receptors metabolism
Phenotype
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26605924
- Full Text :
- https://doi.org/10.1371/journal.pone.0143519