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Ultrasound-Assisted Extraction, Antioxidant and Anticancer Activities of the Polysaccharides from Rhynchosia minima Root.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2015 Nov 23; Vol. 20 (11), pp. 20901-11. Date of Electronic Publication: 2015 Nov 23. - Publication Year :
- 2015
-
Abstract
- Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides from Rhynchosia minima root (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions were also measured. The results showed that optimal extraction parameters were as follows: ultrasound exposure time, 21 min; ratio of water to material, 46 mL/g; ultrasound extraction temperature, 63 °C. Under these conditions, the maximum yield of PRM was 16.95%±0.07%. Furthermore, the main monosaccharides of purified fractions were Ara and Gal. PRM3 and PRM5 exhibited remarkable DPPH radical scavenging activities and reducing power in vitro. PRM3 showed strong inhibitory activities on the growth of MCF-7 cells in vitro. The above results indicate that polysaccharides from R. minima root have the potential to be developed as natural antioxidants and anticancer ingredients for the food and pharmaceutical industries.
- Subjects :
- Antineoplastic Agents, Phytogenic isolation & purification
Antineoplastic Agents, Phytogenic pharmacology
Antioxidants isolation & purification
Antioxidants pharmacology
Cell Survival drug effects
Free Radicals antagonists & inhibitors
Humans
MCF-7 Cells
Polysaccharides isolation & purification
Polysaccharides pharmacology
Antineoplastic Agents, Phytogenic chemistry
Antioxidants chemistry
Fabaceae chemistry
Liquid-Liquid Extraction methods
Plant Roots chemistry
Polysaccharides chemistry
Ultrasonic Waves
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 20
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 26610456
- Full Text :
- https://doi.org/10.3390/molecules201119734