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Simultaneous quantification of ten constituents of Xanthoceras sorbifolia Bunge using UHPLC-MS methods and evaluation of their radical scavenging, DNA scission protective, and α-glucosidase inhibitory activities.
- Source :
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Chinese journal of natural medicines [Chin J Nat Med] 2015 Nov; Vol. 13 (11), pp. 873-880. - Publication Year :
- 2015
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Abstract
- The present study was designed to investigate the bioactive constituents of Xanthoceras sorbifolia in terms of amounts and their antioxidant, DNA scission protection, and α-glucosidase inhibitory activities. Simultaneous quantification of 10 X. sorbifolia constituents was carried out by a newly established ultra-high performance liquid chromatography-quadrupole mass spectrometry method (UHPLC-MS). The antioxidant activities were evaluated by measuring DPPH radical scavenging and DNA scission protective activities. The α-glucosidase inhibitory activities were investigated by using an assay with α-glucosidase from Bacillus Stearothermophilus and disaccharidases from mouse intestine. We found that the wood of X. sorbifolia was rich in phenolic compounds with the contents of catechin, epicatechin, myricetin, and dihydromyricetin being 0.12-0.19, 1.94-2.16, 0.77-0.91, and 6.76-7.89 mg·g(-1), respectively. The four constituents strongly scavenged DPPH radicals (with EC50 being 4.2, 3.8 and 5.7 μg·mL(-1), respectively) and remarkably protected peroxyl radical-induced DNA strand scission (92.10%, 94.66%, 75.44% and 89.95% of protection, respectively, at a concentration of 10 μmol·L(-1)). A dimeric flavan 3-ol, epigallocatechin-(4β→8, 2β→O-7)-epicatechin potently inhibited α-glucosidase with an IC50 value being as low as 1.2 μg·mL(-1). The established UHPLC-MS method could serve as a quality control tool for X. sorbifolia. In conclusion, the high contents of antioxidant and α-glucosidase inhibitory constituents in X. sorbifolia support its use as complementation of other therapeutic agents for metabolic disorders, such as diabetes and hypertension.<br /> (Copyright © 2015 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Antioxidants analysis
Biphenyl Compounds metabolism
Catechin analogs & derivatives
Catechin analysis
Catechin pharmacology
Chromatography, High Pressure Liquid
Flavonoids analysis
Flavonoids pharmacology
Glycoside Hydrolase Inhibitors analysis
Mass Spectrometry
Picrates metabolism
Plant Extracts chemistry
Triterpenes analysis
Triterpenes pharmacology
Wood chemistry
Antioxidants pharmacology
DNA drug effects
DNA Damage
Glycoside Hydrolase Inhibitors pharmacology
Plant Extracts pharmacology
Sapindaceae chemistry
alpha-Glucosidases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1875-5364
- Volume :
- 13
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Chinese journal of natural medicines
- Publication Type :
- Academic Journal
- Accession number :
- 26614463
- Full Text :
- https://doi.org/10.1016/S1875-5364(15)30092-3