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Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product.
- Source :
-
Journal of neuroimmunology [J Neuroimmunol] 2015 Dec 15; Vol. 289, pp. 121-9. Date of Electronic Publication: 2015 Oct 28. - Publication Year :
- 2015
-
Abstract
- The latest therapeutic approaches to Alzheimer disease are using intravenous immunoglobulin (IVIG) products. Therefore, the detailed characterization of target-specific antibodies naturally occurring in IVIG products is beneficial. We have focused on characterization of antibodies isolated against tau protein, a biomarker of Alzheimer's disease, from Flebogamma IVIG product. The analysis of IgG subclass distribution indicated skewing toward IgG3 in anti-tau-enriched IgG fraction. The evaluation of their reactivity and avidity with several recombinant tau forms was performed by ELISA and blotting techniques. Truncated non-phosphorylated tau protein (amino acids 155-421) demonstrated the highest reactivity and avidity index. We provide the first detailed insight into the reactivity of isolated natural antibodies against tau protein.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Epitopes chemistry
Humans
Immunoglobulin G immunology
Immunoglobulins, Intravenous immunology
Immunosorbent Techniques
Molecular Weight
Peptides immunology
Protein Binding immunology
Protein Structure, Tertiary
Immunoglobulin G isolation & purification
Immunoglobulins, Intravenous chemistry
tau Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8421
- Volume :
- 289
- Database :
- MEDLINE
- Journal :
- Journal of neuroimmunology
- Publication Type :
- Academic Journal
- Accession number :
- 26616881
- Full Text :
- https://doi.org/10.1016/j.jneuroim.2015.10.017