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Down-regulation of microRNA-135b inhibited growth of cervical cancer cells by targeting FOXO1.
- Source :
-
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2015 Sep 01; Vol. 8 (9), pp. 10294-304. Date of Electronic Publication: 2015 Sep 01 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- More and more evidence has confirmed that dysregulation of microRNAs (miRNAs) can conduce to the progression of human cancers. Previous studied have shown that dysregulation of miR-135b is in varieties of tumors. However, the roles of miR-135b in cervical cancer remain unknown. Therefore, our aim of this study was to explore the biological function and molecular mechanism of miR-135b in cervical cancer cell lines, discussing whether it could be a therapeutic biomarker of cervical cancer in the future. The MTT assay and ELISA-Brdu assay were used to assess cell proliferation. Cell cycle was detected by flow cytometry. Real-time quantitative polymerase chain reaction (PCR) and Western blot analyses were used to detect expressions of cyclin D1, p21, p27 and FOXO1. In our study, we found that miR-135b is up-regulated in cervical cancer cell lines. Down-regulation of miR-135b evidently inhibited proliferation and arrested cell cycle in cervical cancer cells. Bioinformatics analysis predicted that the FOXO1 was a potential target gene of miR-135b. Besides, miR-135b inhibition significantly increased expressions of the cyclin-dependent kinase inhibitors, p21(/CIP1) and p27(/KIP1), and decreased expression of cyclin D1. However, the high level of miR-135b was associated with increased expression of FOXO1 in cervical cancer cells. Further study by luciferase reporter assay demonstrated that miR-135b could directly target FOXO1. Down-regulation of FOXO1 in cervical cancer cells transfected with miR-135b inhibitor partially reversed its inhibitory effects. In conclusion, down-regulation of miR-135b inhibited cell growth in cervical cancer cells by up-regulation of FOXO1.
- Subjects :
- Cell Cycle genetics
Cell Line, Tumor
Female
Forkhead Box Protein O1
Forkhead Transcription Factors genetics
Humans
MicroRNAs genetics
Up-Regulation
Uterine Cervical Neoplasms genetics
Uterine Cervical Neoplasms pathology
Cell Proliferation genetics
Down-Regulation
Forkhead Transcription Factors metabolism
Gene Expression Regulation, Neoplastic
MicroRNAs metabolism
Uterine Cervical Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1936-2625
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- International journal of clinical and experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 26617737