Back to Search
Start Over
Multimodal In Vivo Imaging of Tumorigenesis and Response to Chemotherapy in a Transgenic Mouse Model of Mammary Cancer.
- Source :
-
Molecular imaging and biology [Mol Imaging Biol] 2016 Aug; Vol. 18 (4), pp. 617-26. - Publication Year :
- 2016
-
Abstract
- Purpose: Transgenic mice expressing the polyoma middle T oncoprotein (PyMT) in the mammary epithelium were explored by multimodal imaging to monitor longitudinally spontaneous tumor growth and response to chemotherapy.<br />Procedures: Positron emission tomography (PET) with 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) and 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT), single photon emission tomography (SPECT) with [(99m)Tc]TcO4 ([(99m)Tc]TEC), X-ray computed tomography, and fluorescent confocal endomicroscopy (FCE) images were acquired during tumor progression in female PyMT mice. Imaging with [(18)F]FDG and [(99m)Tc]TEC was also performed in untreated, doxorubicin-treated, and docetaxel-treated PyMT mice. Total tumor volumes were quantified. Tumors were collected and macroscopic and histological examinations were performed.<br />Results: All PyMT mice developed multifocal tumors of the mammary epithelium that became palpable at 8 weeks of age (W8). Computed tomography (CT) detected tumors at W14, while a clear tumoral uptake of [(99m)Tc]TEC and [(18)F]FDG was present as early as W6 and W8, respectively. No contrast between mammary tumors and surrounding tissue was observed at any stage with [(18)F]FLT. FCE detected an angiogenic switch at W10. Lung metastases were not clearly evidenced by imaging. Doxorubicin and docetaxel treatments delayed tumor growth, as shown by [(18)F]FDG and [(99m)Tc]TEC, but tumor growth resumed upon treatment discontinuation. Tumor growth fitted an exponential model with time constant rates of 0.315, 0.145, and 0.212 week(-1) in untreated, doxorubicin, and docetaxel groups, respectively.<br />Conclusions: Molecular imaging of mammary tumors in PyMT is precocious, precise, and predictive. [(18)F]FDG-PET and [(99m)Tc]TEC SPECT monitor tumor response to chemotherapy.
- Subjects :
- Animals
Antineoplastic Agents pharmacology
Cell Proliferation
Disease Models, Animal
Disease Progression
Female
Fluorescence
Fluorodeoxyglucose F18
Ki-67 Antigen metabolism
Mammary Neoplasms, Animal blood supply
Mammary Neoplasms, Animal diagnostic imaging
Mammary Neoplasms, Animal pathology
Mice
Mice, Transgenic
Technetium chemistry
Time Factors
Tomography, Emission-Computed, Single-Photon
Tomography, X-Ray Computed
Antineoplastic Agents therapeutic use
Carcinogenesis pathology
Mammary Neoplasms, Animal drug therapy
Multimodal Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1860-2002
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular imaging and biology
- Publication Type :
- Academic Journal
- Accession number :
- 26630973
- Full Text :
- https://doi.org/10.1007/s11307-015-0916-7