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Detection of Circulating BRAF(V600E) in Patients with Papillary Thyroid Carcinoma.
- Source :
-
The Journal of molecular diagnostics : JMD [J Mol Diagn] 2016 Jan; Vol. 18 (1), pp. 100-8. Date of Electronic Publication: 2015 Nov 26. - Publication Year :
- 2016
-
Abstract
- BRAF(V600E) is a common mutation in papillary thyroid carcinoma (PTC) correlated with aggressive features. Our objective was to assess the feasibility and accuracy of a novel RNA-based blood assay to identify individuals with a high-risk tumor mutation in patients with PTC. Patients with benign or malignant thyroid disorders were included between September 2013 and July 2014 before either thyroidectomy (n = 62) or treatment of recurrent or metastatic PTC (n = 8). RNA was isolated from peripheral blood lymphocytes and reverse transcribed and followed by two rounds of nested PCR amplification with a restriction digest specific for wild-type BRAF. BRAF(V600E) levels were quantified with standardization curves. Circulating BRAF(V600E) levels were compared with BRAF mutation status from surgical pathologic DNA-based tissue assays. Testing characteristics and receiving-operator curve using tissue results as the gold standard were assessed. Matched blood and tissue assays for BRAF(V600E) were performed on 70 patients with PTC (stages I to IV, n = 48) or other (n = 22) thyroid tumors. Sixty-three percent of PTC patients tested positive for BRAF(V600E) with conventional tissue assays on surgical specimens. The correlation between the RNA-based blood assay and tissue BRAF status was 0.71. PTC patients harbor detectable BRAF(V600E) circulating tumor cells. This blood assay is feasible and has potential as a biomarker for prognosis, surveillance, clinical decision making, and assessment of treatment response to BRAF-targeted therapies.<br /> (Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Carcinoma surgery
Carcinoma, Papillary
Female
Humans
Lymphocytes cytology
Male
Middle Aged
Mutation genetics
Reverse Transcriptase Polymerase Chain Reaction
Thyroid Cancer, Papillary
Thyroid Neoplasms surgery
Thyroidectomy
Biomarkers, Tumor blood
Biomarkers, Tumor genetics
Carcinoma blood
Carcinoma genetics
Proto-Oncogene Proteins B-raf blood
Proto-Oncogene Proteins B-raf genetics
Thyroid Neoplasms blood
Thyroid Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1943-7811
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of molecular diagnostics : JMD
- Publication Type :
- Academic Journal
- Accession number :
- 26631873
- Full Text :
- https://doi.org/10.1016/j.jmoldx.2015.08.003