Application of succulent plant leaves for Agrobacterium infiltration-mediated protein production.

Authors :: Jones RW
Source :: Journal of microbiological methods [J Microbiol Methods] 2016 Jan; Vol. 120, pp. 65-7. Date of Electronic Publication: 2015 Dec 02.
Publication Year :: 2016
Abstract: When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.<br /> (Published by Elsevier B.V.)

Subjects :: Agrobacterium enzymology
Agrobacterium genetics
Agrobacterium growth & development
Bacterial Proteins biosynthesis
Bacterial Proteins genetics
Crassulaceae metabolism
Genes, Plant
Glycoside Hydrolases antagonists & inhibitors
Glycoside Hydrolases biosynthesis
Glycoside Hydrolases genetics
Plants, Genetically Modified
Plasmids genetics
Sedum microbiology
Nicotiana microbiology
Agrobacterium physiology
Kalanchoe microbiology
Plant Leaves microbiology

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