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MicroRNA-9 Mediates the Cell Apoptosis by Targeting Bcl2l11 in Ischemic Stroke.
- Source :
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Molecular neurobiology [Mol Neurobiol] 2016 Dec; Vol. 53 (10), pp. 6809-6817. Date of Electronic Publication: 2015 Dec 11. - Publication Year :
- 2016
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Abstract
- Ischemic strokes occur as a result of an obstruction within a blood vessel supplying blood to the brain and accounts for about 87 % of all cases. During the cerebral ischemia, most of the neurons undergo the necrosis and apoptosis upon the exposure to the dramatic blood flow reduction. Although, it is known that both the intrinsic and extrinsic pathways are involved in the neuronal apoptosis of ischemic brain injury. The complex underlying mechanisms remains less known. MicroRNAs are a class of endogenous small non-coding RNAs and the role of miRNAs in the pathophysiology of stroke has been studied. In this study, we found that miR-9 is downregulated in the mice with middle cerebral artery occlusion (MCAO) brain and oxygen-glucose deprivation (OGD) neurons. Application of miR-9 gamer could restore the neurological scores and reduces the infarct volume, brain water content, and the behavioral impairments. Moreover, upregulation of miR-9 suppresses the neuronal apoptosis in MCAO brain and OGD neurons. Furthermore, we identified that Bcl2l11 as the direct target of miR-9 and manipulation of miR-9 induces the corresponding changing of Bcl2l11 protein level. Finally, we found that the protein level of Bcl2l11 is increased in the MCAO brain and OGD neurons. Our study demonstrated the critical role of miR-9 in the neuronal apoptosis of ischemic brain injury.
- Subjects :
- Animals
Base Sequence
Behavior, Animal
Brain Ischemia complications
Disease Models, Animal
Glucose deficiency
Infarction, Middle Cerebral Artery complications
Infarction, Middle Cerebral Artery pathology
Mice, Inbred C57BL
Neurons metabolism
Neurons pathology
Oxygen
Protein Biosynthesis
Stroke complications
Up-Regulation genetics
Apoptosis genetics
Bcl-2-Like Protein 11 metabolism
Brain Ischemia genetics
Brain Ischemia pathology
MicroRNAs metabolism
Stroke genetics
Stroke pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1182
- Volume :
- 53
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 26660116
- Full Text :
- https://doi.org/10.1007/s12035-015-9605-4