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MicroRNA-144 mediates metabolic shift in ovarian cancer cells by directly targeting Glut1.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2016 May; Vol. 37 (5), pp. 6855-60. Date of Electronic Publication: 2015 Dec 11. - Publication Year :
- 2016
-
Abstract
- Warburg effect is characterized by an increased utilization of glucose via glycolysis in cancer cells, even when enough oxygen is present to properly respire. Recent studies demonstrate that deregulation of microRNAs contributes to the Warburg effect. In the present study, we show that miR-144 is downregulated while glucose transporter 1 (Glut1) is upregulated in ovarian cancers. In vitro studies further showed that miR-144 inhibits Glut1 expression through targeting its 3'-untranslated region. As a result, cells overexpressing miR-144 exhibited a metabolic shift, including enhanced glucose uptake and lactate production. The altered glucose metabolism induced by miR-144 also leads to a rapid growth of ovarian cancer cells. Taken together, our results indicate that miR-144 may serve as a molecular switch to regulate glycolysis in ovarian cancer by targeting the expression of Glut1.
- Subjects :
- 3' Untranslated Regions
Animals
Binding Sites
Cell Line, Tumor
Cell Proliferation
Disease Models, Animal
Down-Regulation
Energy Metabolism
Female
Glucose metabolism
Glycolysis
Heterografts
Humans
Male
Mice
Ovarian Neoplasms pathology
Tumor Burden
Gene Expression Regulation, Neoplastic
Glucose Transporter Type 1 genetics
MicroRNAs genetics
Ovarian Neoplasms genetics
Ovarian Neoplasms metabolism
RNA Interference
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 37
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26662316
- Full Text :
- https://doi.org/10.1007/s13277-015-4558-9