Back to Search
Start Over
Toxic effect of NiCl2 on development of the bursa of Fabricius in broiler chickens.
- Source :
-
Oncotarget [Oncotarget] 2016 Jan 05; Vol. 7 (1), pp. 125-39. - Publication Year :
- 2016
-
Abstract
- This study was conducted with objective of evaluating the toxic effects of nickel chloride (NiCl2) on development of bursa of Fabricius in broilers fed on diets supplemented with 0, 300, 600 and 900 mg/kg of NiCl2 for 42 days by using the methods of experimental pathology, flow cytometry (FCM), and quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that dietary NiCl2 in 300 mg/kg and over induced toxic suppression in the bursal development, which was characterized by decreasing lymphocytes histopathologically and relative weight, increasing G0/G1 phase (a prolonged nondividing state), reducing S phase (DNA replication) and proliferating index, and increasing percentages of apoptotic cells. Concurrently, the mRNA expression levels of bax, cytochrome c (cyt c), apoptotic peptidase activating factor 1 (Apaf-1), caspase-3, caspase-6, caspase-7 and caspase-9 were increased and the bcl-2 mRNA expression levels were decreased. The toxic suppression of bursal development finally impaired humoral immunity duo to the reduction of B lymphocyte population and B lymphocyte activity in the broiler chicken. This study provides new evidences for further studying the effect mechanism of Ni and Ni compoundson B-cell or bursa of Fabricius.
- Subjects :
- Animals
Apoptosis genetics
Apoptosis Regulatory Proteins genetics
Avian Proteins genetics
B-Lymphocytes metabolism
Body Weight drug effects
Body Weight genetics
Bursa of Fabricius growth & development
Bursa of Fabricius metabolism
Caspases genetics
Cell Cycle drug effects
Cell Cycle genetics
Cell Proliferation drug effects
Cell Proliferation genetics
Chickens
Cytochromes c genetics
Lymphocyte Count
Organ Size drug effects
Organ Size genetics
Reverse Transcriptase Polymerase Chain Reaction
bcl-2-Associated X Protein genetics
Apoptosis drug effects
B-Lymphocytes drug effects
Bursa of Fabricius drug effects
Gene Expression Regulation, Developmental drug effects
Nickel toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1949-2553
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oncotarget
- Publication Type :
- Academic Journal
- Accession number :
- 26683707
- Full Text :
- https://doi.org/10.18632/oncotarget.6591