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Visualization and quantification of Plasmodium falciparum intraerythrocytic merozoites.
- Source :
-
Systems and synthetic biology [Syst Synth Biol] 2015 Dec; Vol. 9 (Suppl 1), pp. 23-6. Date of Electronic Publication: 2015 Apr 05. - Publication Year :
- 2015
-
Abstract
- Malaria, a leading parasitic killer, is caused by Plasmodium spp. The pathology of the disease starts when Plasmodium merozoites infect erythrocytes to form rings, that matures through a large trophozoite form and develop into schizonts containing multiple merozoites. The number of intra-erythrocytic merozoites is a key-determining factor for multiplication rate of the parasite. Counting of intraerythrocytic merozoites by classical 2-D microscopy method is error prone due to insufficient representation of merozoite in one optical plane of a schizont. Here, we report an alternative 3-D microscopy based automated method for counting of intraerythrocytic merozoites in entire volume of schizont. This method offers a considerable amount of advantages in terms of both, ease and accuracy.
Details
- Language :
- English
- ISSN :
- 1872-5325
- Volume :
- 9
- Issue :
- Suppl 1
- Database :
- MEDLINE
- Journal :
- Systems and synthetic biology
- Publication Type :
- Academic Journal
- Accession number :
- 26702305
- Full Text :
- https://doi.org/10.1007/s11693-015-9167-9