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Thermoresponsive amperometric glucose biosensor.

Authors :
Pinyou P
Ruff A
Pöller S
Barwe S
Nebel M
Alburquerque NG
Wischerhoff E
Laschewsky A
Schmaderer S
Szeponik J
Plumeré N
Schuhmann W
Source :
Biointerphases [Biointerphases] 2015 Mar 24; Vol. 11 (1), pp. 011001. Date of Electronic Publication: 2015 Mar 24.
Publication Year :
2015

Abstract

The authors report on the fabrication of a thermoresponsive biosensor for the amperometric detection of glucose. Screen printed electrodes with heatable gold working electrodes were modified by a thermoresponsive statistical copolymer [polymer I: poly(ω-ethoxytriethylenglycol methacrylate-co-3-(N,N-dimethyl-N-2-methacryloyloxyethyl ammonio) propanesulfonate-co-ω-butoxydiethylenglycol methacrylate-co-2-(4-benzoyl-phenoxy)ethyl methacrylate)] with a lower critical solution temperature of around 28 °C in aqueous solution via electrochemically induced codeposition with a pH-responsive redox-polymer [polymer II: poly(glycidyl methacrylate-co-allyl methacrylate-co-poly(ethylene glycol)methacrylate-co-butyl acrylate-co-2-(dimethylamino)ethyl methacrylate)-[Os(bpy)2(4-(((2-(2-(2-aminoethoxy)ethoxy)ethyl)amino)methyl)-N,N-dimethylpicolinamide)](2+)] and pyrroloquinoline quinone-soluble glucose dehydrogenase acting as biological recognition element. Polymer II bears covalently bound Os-complexes that act as redox mediators for shuttling electrons between the enzyme and the electrode surface. Polymer I acts as a temperature triggered immobilization matrix. Probing the catalytic current as a function of the working electrode temperature shows that the activity of the biosensor is dramatically reduced above the phase transition temperature of polymer I. Thus, the local modulation of the temperature at the interphase between the electrode and the bioactive layer allows switching the biosensor from an on- to an off-state without heating of the surrounding analyte solution.

Details

Language :
English
ISSN :
1559-4106
Volume :
11
Issue :
1
Database :
MEDLINE
Journal :
Biointerphases
Publication Type :
Academic Journal
Accession number :
26702635
Full Text :
https://doi.org/10.1116/1.4938382