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[Effect of poly(ADP-ribose)polymerase inhibitor combined with carbo on apoptosis of human breast cancer cells].
- Source :
-
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences [Zhejiang Da Xue Xue Bao Yi Xue Ban] 2015 Sep; Vol. 44 (5), pp. 506-10. - Publication Year :
- 2015
-
Abstract
- Objective: To investigate the effect of poly(ADP-ribose)polymerase(PARP)inhibitor ABT888 combined with carbo on apoptosis of human breast cancer cells.<br />Methods: MTT was used to detect the cell viability of MDA-MB-435s cells after treatment of carbo and ABT888 with different concentration. FACS and Western-blotting were used to detect the cell apoptosis rate and apoptosis-related protein expression, respectively.<br />Results: Combined application of carbo and ABT888 significantly inhibited the proliferation of MDA-MB-435s cells, and the inhibition rates were significantly higher than that of carbo or ABT888 alone. The combination of carbo and ABT888 markedly induced cell apoptosis(26.3%±1.5%) more than carbo(18.6%±1.6%, P<0.01) and ABT888(14.7%±2.3%, P<0.01) alone. Combination of carbo and ABT888 significantly down-regulated the expression of anti-apoptosis factors Bcl-2 and up-regulated the pro-apoptosis proteins Bax and cleaved caspase-3.<br />Conclusion: The combination of carbo and ABT888 can suppress the proliferation and induce apoptosis of human breast cancer DA-MB-435s cells.
- Subjects :
- Caspase 3 metabolism
Cell Line, Tumor drug effects
Cell Survival
Humans
Proto-Oncogene Proteins c-bcl-2 metabolism
bcl-2-Associated X Protein metabolism
Apoptosis
Benzimidazoles pharmacology
Breast Neoplasms pathology
Carboplatin pharmacology
Poly(ADP-ribose) Polymerase Inhibitors pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1008-9292
- Volume :
- 44
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 26713524
- Full Text :
- https://doi.org/10.3785/j.issn.1008-9292.2015.09.06