Back to Search
Start Over
Simultaneous determination of loganin, morroniside, catalpol and acteoside in normal and chronic kidney disease rat plasma by UPLC-MS for investigating the pharmacokinetics of Rehmannia glutinosa and Cornus officinalis Sieb drug pair extract.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2016 Jan 15; Vol. 1009-1010, pp. 122-9. Date of Electronic Publication: 2015 Dec 14. - Publication Year :
- 2016
-
Abstract
- A sensitive and rapid method for determination of loganin, morroniside, catalpol and acteoside in rat plasma after oral administration of Rehmannia glutinosa Libosch and Cornus officinalis Sieb drug pair based on ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS). Chromatographic separation was achieved using an Acquity UPLC BEH C18 column (100mm×2.1mm, 1.7μm) at a flow rate of 0.4mL/min, using gradient mode containing 0.1% formic acid in water and acetonitrile were used as the mobile phase A and B. Loganin, morroniside, catalpol, acteoside and the internal standard (chloramphenicol) were detected by selected reaction monitoring in the negative ion mode with the mass transition of m/z 451.0→179.0 (morroniside), m/z 435.0→227.0 (loganin), m/z 407.1→199.1 (catalpol), m/z 623.2→161.0 (acteoside) and m/z 320.8→151.9 (chloramphenicol), respectively. All calibration curves showed good linearity (r>0.991). The precision was evaluated by intra-day and inter-day assays and the RSD% were all within 9.58%. The recovery ranged from 67.62 to 80.14%. The method was successfully applied to pharmacokinetic study of the analytes in normal and doxorubicin-induced chronic kidney disease rat plasma.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Oral
Animals
Chromatography, High Pressure Liquid
Cornus chemistry
Drugs, Chinese Herbal administration & dosage
Drugs, Chinese Herbal chemistry
Glucosides chemistry
Glycosides chemistry
Iridoid Glucosides chemistry
Iridoids chemistry
Limit of Detection
Male
Mass Spectrometry
Phenols chemistry
Rats
Rats, Sprague-Dawley
Rehmannia chemistry
Drugs, Chinese Herbal pharmacokinetics
Glucosides blood
Glycosides blood
Iridoid Glucosides blood
Iridoids blood
Phenols blood
Renal Insufficiency, Chronic blood
Subjects
Details
- Language :
- English
- ISSN :
- 1873-376X
- Volume :
- 1009-1010
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 26720701
- Full Text :
- https://doi.org/10.1016/j.jchromb.2015.12.020