Back to Search
Start Over
Dipyridamole attenuates ischemia reperfusion induced acute kidney injury through adenosinergic A1 and A2A receptor agonism in rats.
Dipyridamole attenuates ischemia reperfusion induced acute kidney injury through adenosinergic A1 and A2A receptor agonism in rats.
- Source :
-
Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2016 Apr; Vol. 389 (4), pp. 361-8. Date of Electronic Publication: 2016 Jan 04. - Publication Year :
- 2016
-
Abstract
- Dipyridamole (DYP) is an anti-platelet agent with marked vasodilator, anti-oxidant, and anti-inflammatory activity. The present study investigated the role of adenosine receptors in DYP-mediated protection against ischemia reperfusion-induced acute kidney injury (AKI) in rats. The rats were subjected to bilateral renal ischemia for 40 min followed by reperfusion for 24 h. The renal damage induced by ischemia reperfusion injury (IRI) was assessed by measuring creatinine clearance, blood urea nitrogen, uric acid, plasma potassium, fractional excretion of sodium, and microproteinuria in rats. The oxidative stress in renal tissues was assessed by quantification of thiobarbituric acid-reactive substances, superoxide anion generation, and reduced glutathione level. The hematoxylin-eosin staining was carried out to observe histopathological changes in renal tissues. DYP (10 and 30 mg/kg, intraperitoneal, i.p.) was administered 30 min before subjecting the rats to renal IRI. In separate groups, caffeine (50 mg/kg, i.p.), an adenosinergic A1 and A2A receptor antagonist was administered with and without DYP treatment before subjecting the rats to renal IRI. The ischemia reperfusion-induced AKI was demonstrated by significant changes in serum as well as urinary parameters, enhanced oxidative stress, and histopathological changes in renal tissues. The administration of DYP demonstrated protection against AKI. The prior treatment with caffeine abolished DYP-mediated reno-protection suggesting role of A1 and A2A adenosine receptors in DYP-mediated reno-protection in rats. It is concluded that adenosine receptors find their definite involvement in DYP-mediated anti-oxidative and reno-protective effect against ischemia reperfusion-induced AKI.
- Subjects :
- Acute Kidney Injury metabolism
Acute Kidney Injury pathology
Adenosine A1 Receptor Antagonists pharmacology
Adenosine A2 Receptor Antagonists pharmacology
Animals
Biomarkers blood
Biomarkers urine
Caffeine pharmacology
Cytoprotection
Disease Models, Animal
Kidney metabolism
Male
Oxidative Stress drug effects
Rats, Wistar
Receptor, Adenosine A1 metabolism
Receptor, Adenosine A2A metabolism
Reperfusion Injury metabolism
Reperfusion Injury pathology
Signal Transduction
Acute Kidney Injury prevention & control
Adenosine A1 Receptor Agonists pharmacology
Adenosine A2 Receptor Agonists pharmacology
Dipyridamole pharmacology
Kidney pathology
Receptor, Adenosine A1 drug effects
Receptor, Adenosine A2A drug effects
Reperfusion Injury prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1912
- Volume :
- 389
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Naunyn-Schmiedeberg's archives of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26728617
- Full Text :
- https://doi.org/10.1007/s00210-015-1206-2