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Rice phytochrome-interacting factor protein OsPIF14 represses OsDREB1B gene expression through an extended N-box and interacts preferentially with the active form of phytochrome B.
Rice phytochrome-interacting factor protein OsPIF14 represses OsDREB1B gene expression through an extended N-box and interacts preferentially with the active form of phytochrome B.
- Source :
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Biochimica et biophysica acta [Biochim Biophys Acta] 2016 Feb; Vol. 1859 (2), pp. 393-404. Date of Electronic Publication: 2015 Dec 28. - Publication Year :
- 2016
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Abstract
- DREB1/CBF genes, known as major regulators of plant stress responses, are rapidly and transiently induced by low temperatures. Using a yeast one-hybrid screening, we identified a putative Phytochrome-Interacting bHLH Factor (OsPIF14), as binding to the OsDREB1B promoter. bHLH proteins are able to bind to hexameric E-box (CANNTG) or N-box (CACG(A/C)G) motifs, depending on transcriptional activity. We have shown that OsPIF14 binds to the OsDREB1B promoter through two N-boxes and that the flanking regions of the hexameric core are essential for protein-DNA interaction and stability. We also showed that OsPIF14 down-regulates OsDREB1B gene expression in rice protoplasts, corroborating the OsPIF14 repressor activity observed in the transactivation assays using Arabidopsis protoplasts. In addition, we showed that OsPIF14 is indeed a phytochrome interacting factor, which preferentially binds to the active form (Pfr) of rice phytochrome B. This raises the possibility that OsPIF14 activity might be modulated by light. However, we did not observe any regulation of the OsDREB1B gene expression by light under control conditions. Moreover, OsPIF14 gene expression was shown to be modulated by different treatments, such as drought, salt, cold and ABA. Interestingly, OsPIF14 showed also a specific cold-induced alternative splicing. All together, these results suggest the possibility that OsPIF14 is involved in cross-talk between light and stress signaling through interaction with the OsDREB1B promoter. Although in the absence of stress, OsDREB1B gene expression was not regulated by light, given previous reports, it remains possible that OsPIF14 has a role in light modulation of stress responses.<br /> (Copyright © 2015. Published by Elsevier B.V.)
- Subjects :
- Amino Acid Sequence genetics
Arabidopsis Proteins metabolism
Cold Temperature
DNA-Binding Proteins genetics
Droughts
Gene Expression Regulation, Plant
Oryza physiology
Phytochrome B metabolism
Plants, Genetically Modified
Promoter Regions, Genetic
Transcription Factors metabolism
Arabidopsis Proteins genetics
Oryza genetics
Phytochrome B genetics
Plant Proteins genetics
Stress, Physiological genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1859
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 26732823
- Full Text :
- https://doi.org/10.1016/j.bbagrm.2015.12.008