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Attractant and repellent cues cooperate in guiding a subset of olfactory sensory axons to a well-defined protoglomerular target.
- Source :
-
Development (Cambridge, England) [Development] 2016 Jan 01; Vol. 143 (1), pp. 123-32. - Publication Year :
- 2016
-
Abstract
- Olfactory sensory axons target well-defined intermediate targets in the zebrafish olfactory bulb called protoglomeruli well before they form odorant receptor-specific glomeruli. A subset of olfactory sensory neurons are labeled by expression of the or111-7:IRES:GAL4 transgene whose axons terminate in the central zone (CZ) protoglomerulus. Previous work has shown that some of these axons misproject to the more dorsal and anterior dorsal zone (DZ) protoglomerulus in the absence of Netrin 1/Dcc signaling. In search of additional cues that guide these axons to the CZ, we found that Semaphorin 3D (Sema3D) is expressed in the anterior bulb and acts as a repellent that pushes them towards the CZ. Further analysis indicates that Sema3D signaling is mediated through Nrp1a, while Nrp2b also promotes CZ targeting but in a Sema3D-independent manner. nrp1a, nrp2b and dcc transcripts are detected in or111-7 transgene-expressing neurons early in development and both Nrp1a and Dcc act cell-autonomously in sensory neurons to promote accurate targeting to the CZ. dcc and nrp1a double mutants have significantly more DZ misprojections than either single mutant, suggesting that the two signaling systems act independently and in parallel to direct a specific subset of sensory axons to their initial protoglomerular target.<br /> (© 2016. Published by The Company of Biologists Ltd.)
- Subjects :
- Animals
Cues
DCC Receptor
DNA-Binding Proteins metabolism
Gene Expression Regulation, Developmental
Nerve Growth Factors genetics
Nerve Growth Factors metabolism
Netrin-1
Neuropilin-1 genetics
Neuropilin-2 genetics
Olfactory Bulb growth & development
Olfactory Pathways physiology
Receptors, Cell Surface genetics
Receptors, Cell Surface metabolism
Semaphorins biosynthesis
Semaphorins genetics
Semaphorins metabolism
Signal Transduction
Transcription Factors metabolism
Tumor Suppressor Proteins genetics
Tumor Suppressor Proteins metabolism
Zebrafish Proteins genetics
Zebrafish Proteins metabolism
Neuropilin-1 metabolism
Neuropilin-2 metabolism
Olfactory Bulb cytology
Olfactory Pathways embryology
Olfactory Receptor Neurons cytology
Zebrafish embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 143
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 26732841
- Full Text :
- https://doi.org/10.1242/dev.127985