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1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay.
- Source :
-
The Analyst [Analyst] 2016 Feb 21; Vol. 141 (4), pp. 1331-8. Date of Electronic Publication: 2016 Jan 07. - Publication Year :
- 2016
-
Abstract
- An alkyl azide derivative of 1-phenylethynylpyrene (PEPy) dye was prepared and used in the functionalization of oligonucleotides via click chemistry. Spectral and photo-physical properties of the PEPy-modified oligonucleotides as a single strand, and in perfect or mismatched duplexes, have been studied. A series of PEPy-Dabcyl fluorogenic TaqMan probes were synthesized and tested in qPCR. PEPy proved to be a superior substitute for AMCA as a short wavelength fluorescent dye for qPCR probes. PEPy probes were shown to significantly reduce Cq (a fractional PCR cycle used for quantification) vs. AMCA labeled probes, thus improving on the reliability of detection. Moreover, a larger increase of fluorescence during amplification was observed in the case of PEPy probes that makes this dye very suitable for an end-point PCR technique. This study broadens the panel of fluorescent dyes suitable for the use in probes for quantitative real-time PCR.
- Subjects :
- Alkynes chemistry
Azides chemistry
Catalysis
Click Chemistry
Color
Copper chemistry
Coumarins chemistry
Fluorescent Dyes chemical synthesis
Oligonucleotides chemistry
Oligonucleotides genetics
Pyrenes chemical synthesis
Fluorescent Dyes chemistry
Pyrenes chemistry
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1364-5528
- Volume :
- 141
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 26738806
- Full Text :
- https://doi.org/10.1039/c5an01767j