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Decreased salivary matrix metalloproteinase-8 reflecting a defensive potential in juvenile parotitis.
- Source :
-
International journal of pediatric otorhinolaryngology [Int J Pediatr Otorhinolaryngol] 2016 Jan; Vol. 80, pp. 74-7. Date of Electronic Publication: 2015 Nov 26. - Publication Year :
- 2016
-
Abstract
- Objective: Matrix metalloproteinases MMP-2 and MMP-9 have been associated with juvenile parotitis. However, the role of MMP-8 has not been addressed previously. This work focuses on salivary MMP-8 and -9 levels in juvenile parotitis.<br />Methods: During a five-year period at Helsinki University Hospital, a tertiary care hospital, 41 patients aged 17 or under, were identified as having parotitis; from 36 of these patients, saliva samples were collected for MMP-8 IFMA (time-resolved immunofluorometric assay) analyses. Control saliva samples were collected from 34 age- and gender-matched children admitted for an elective surgery who had no history of parotitis. For comparison, salivary levels of MMP-9, tissue inhibitor of matrix metalloproteinase (TIMP-1), MMP-8/TIMP-1 ratio, human neutrophil elastase (HNE), and myeloperoxidase (MPO) were analyzed by ELISA. Additionally, salivary MMP-8 levels were compared to historical saliva samples from 18 adult gingivitis patients as well as to 10 healthy adult controls.<br />Results: The median (25%, 75% percentile) MMP-8 concentration in saliva of parotitis patients was significantly lower than MMP-8 concentration in saliva of their controls [50.4ng/ml (37.5, 72.9) vs. 148.5ng/ml (101.2, 178.5) p<0.0001] and lower than in patients with gingivitis [347.9ng/ml (242.6, 383.2) p<0.0001] or healthy adult controls [257.2ng/ml (164.9, 320.7) p<0.0001]. The MMP-8/TIMP-1 ratio was lower than in controls [0.13 (0.05-0.02) vs. 0.3 (0.17-0.46) p<0.0001]. The median MMP-9 concentration in saliva of parotitis patients was significantly higher than in controls [143.9ng/m (68.8-189.0) vs. 34.9ng/ml (16.3-87.6) p<0.0001]. Neither HNE, MPO, nor TIMP-1 alone separated the patients from the control groups.<br />Conclusions: MMP-9 was up-regulated in juvenile parotitis saliva, suggesting that MMP-9 may play a destructive role in juvenile parotitis, as others have suggested. The present novel findings reveal a decreased salivary MMP-8 concentration, suggesting that MMP-8 may reflect in juvenile parotitis down-regulated or anti-inflammatory immune characteristics.<br /> (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Adolescent
Adult
Case-Control Studies
Child
Child, Preschool
Female
Gingivitis enzymology
Humans
Leukocyte Elastase metabolism
Male
Peroxidase metabolism
Tissue Inhibitor of Metalloproteinase-1 metabolism
Matrix Metalloproteinase 8 metabolism
Matrix Metalloproteinase 9 metabolism
Parotitis enzymology
Saliva metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8464
- Volume :
- 80
- Database :
- MEDLINE
- Journal :
- International journal of pediatric otorhinolaryngology
- Publication Type :
- Academic Journal
- Accession number :
- 26746616
- Full Text :
- https://doi.org/10.1016/j.ijporl.2015.11.012