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SEUSS Integrates Gibberellin Signaling with Transcriptional Inputs from the SHR-SCR-SCL3 Module to Regulate Middle Cortex Formation in the Arabidopsis Root.
- Source :
-
Plant physiology [Plant Physiol] 2016 Mar; Vol. 170 (3), pp. 1675-83. Date of Electronic Publication: 2016 Jan 27. - Publication Year :
- 2016
-
Abstract
- A decade of studies on middle cortex (MC) formation in the root endodermis of Arabidopsis (Arabidopsis thaliana) have revealed a complex regulatory network that is orchestrated by several GRAS family transcription factors, including SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE3 (SCL3). However, how their functions are regulated remains obscure. Here we show that mutations in the SEUSS (SEU) gene led to a higher frequency of MC formation. seu mutants had strongly reduced expression of SHR, SCR, and SCL3, suggesting that SEU positively regulates these genes. Our results further indicate that SEU physically associates with upstream regulatory sequences of SHR, SCR, and SCL3; and that SEU has distinct genetic interactions with these genes in the control of MC formation, with SCL3 being epistatic to SEU. Similar to SCL3, SEU was repressed by the phytohormone GA and induced by the GA biosynthesis inhibitor paclobutrazol, suggesting that SEU acts downstream of GA signaling to regulate MC formation. Consistently, we found that SEU mediates the regulation of SCL3 by GA signaling. Together, our study identifies SEU as a new critical player that integrates GA signaling with transcriptional inputs from the SHR-SCR-SCL3 module to regulate MC formation in the Arabidopsis root.<br /> (© 2016 American Society of Plant Biologists. All Rights Reserved.)
- Subjects :
- Arabidopsis cytology
Arabidopsis genetics
Arabidopsis Proteins genetics
Co-Repressor Proteins genetics
Epistasis, Genetic
Gene Expression Regulation, Plant
Microscopy, Confocal
Models, Genetic
Mutation
Plant Roots cytology
Plant Roots genetics
Plants, Genetically Modified
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Transcription Factors genetics
Two-Hybrid System Techniques
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Co-Repressor Proteins metabolism
Gibberellins metabolism
Plant Roots metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 170
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 26818732
- Full Text :
- https://doi.org/10.1104/pp.15.01501